Home LiteratureArticle Details
PMID: 340898 Published · ppublish English Journal Article

Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light.

Molecular & general genetics : MGG ·Vol. 156 ·No. 2 ·1977-11-14 ·Pages 121-31

Kato T, Shinoura Y

Abstract

Mutants of E. coli defective in susceptibility to UV-induction of mutations were isolated by direct screening for their UV nonmutable phenotype (Umu-). Screening of about 30,000 mutagenized clones of a uvr-B derivative of AB1157 yielded six Umu- strains. The mutants can be classified into three groups by the location of the mutations, umuA, umuB and umuC. Mutations umuA and umuB are, respectively, mapped close to lexA and recA genes and mutations at both loci partially reduce UV mutagenesis. The locus of umuC is between hemA and purB and the mutations at this new locus result in a moderate increase of UV sensitivity. The mutation diminishes UV mutagenesis and UV reactivation of phage lambda without affecting the inducibility of phophage lambda nor the inhibition of cell division following UV irradiation. Related properties of an isogenic strain of a recF- mutant are compared with those of umuC-.

MeSH Terms
Cell Division/radiation effects Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,isolation & purification,radiation effects Genes Lysogeny Mutation Phenotype Radiation Tolerance Ultraviolet Rays
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kato T
Shinoura Y
References (39)
39 references, click to expand
  1. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.
    Proc Natl Acad Sci U S A. 1964 Feb;51:226-31 PMID: 14124320
  2. Photoreactivation of mutation and killing in Escherichia coli.
    Adv Biol Med Phys. 1968;12:283-98 PMID: 4879501
  3. The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12.
    Mol Gen Genet. 1975 Dec 1;141(3):189-205 PMID: 1107815
  4. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  5. Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations.
    J Bacteriol. 1972 Nov;112(2):886-93 PMID: 4343824
  6. Studies on radiation-sensitive mutants of E. coli. 3. Participation of the rec system in induction of mutation by ultraviolet irradiation.
    Mol Gen Genet. 1968;103(1):1-10 PMID: 4890247
  7. Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation.
    J Mol Biol. 1970 Aug;51(3):459-72 PMID: 4923857
  8. Effect of ultraviolet irradiation on bacteriophage lambda immunity.
    J Mol Biol. 1967 Jan 28;23(2):247-63 PMID: 5340187
  9. [Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation].
    Mol Gen Genet. 1972;119(2):153-74 PMID: 4577433
  10. Spontaneous and radiation-induced deletion mutations in Escherichia coli strains with different DNA repair capacities.
    Mutat Res. 1972 Sep;16(7):13-25 PMID: 4559755
  11. Evidence for the control by exrA and polA genes of two branches of the uvr gene-dependent excision repair pathway in Escherichia coli K-12.
    J Bacteriol. 1973 Oct;116(1):175-82 PMID: 4583206
  12. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.
    Proc Natl Acad Sci U S A. 1965 Feb;53:451-9 PMID: 14294081
  13. Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation.
    Genetics. 1977 Sep;87(1):1-18 PMID: 334628
  14. Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli.
    Mol Gen Genet. 1969;105(4):285-97 PMID: 4904136
  15. Elevated mutability of polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system ("SOS repair") and its anomalous expression in these strains.
    Genetics. 1975 Jun;79 Suppl:199-213 PMID: 1097302
  16. Effects of chloramphenicol and caffeine on postreplication repair in uvr A- umuC- und uvrA- recF- strains of Escherichia coli K-12.
    Mol Gen Genet. 1977 Nov 14;156(2):115-20 PMID: 340897
  17. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif.
    Mol Gen Genet. 1975 Oct 22;140(4):309-332 PMID: 1107802
  18. IexB: a new gene governing radiation sensitivity and lysogenic induction in Escherichia coli K12.
    Basic Life Sci. 1975;5A:379-82 PMID: 1103847
  19. Proteolytic cleavage of bacteriophage lambda repressor in induction.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51 PMID: 1090931
  20. [Induction of the production of bacteriophages in lysogenic bacteria].
    Ann Inst Pasteur (Paris). 1950 Dec;79(6):815-59 PMID: 14811113
  21. Respiration, growth and viability of repair-deficient mutants of Escherichia coli after ultraviolet irradiation.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1974 Jan;25(1):51-60 PMID: 4588877
  22. Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation.
    Mol Gen Genet. 1972;119(2):139-52 PMID: 4565754
  23. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  24. The beginning of an investigation of the role of recF in the pathways of metabolism of ultraviolet-irradiated DNA in Escherichia coli.
    Basic Life Sci. 1975;5A:283-91 PMID: 1103834
  25. Pleiotropic effect of the rec A gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication.
    J Bacteriol. 1971 May;106(2):539-42 PMID: 4929866
  26. Induction of Mutations in a Bacterial Virus.
    Proc Natl Acad Sci U S A. 1953 Jul;39(7):628-36 PMID: 16589315
  27. Suppression of lex mutations affecting deoxyribonucleic acid repair in Escherichia coli K-12 by closely linked thermosensitive mutations.
    J Bacteriol. 1973 Nov;116(2):950-6 PMID: 4583257
  28. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.
    Proc Natl Acad Sci U S A. 1964 Feb;51:293-300 PMID: 14124327
  29. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  30. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  31. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants.
    J Mol Biol. 1973 Oct 25;80(2):327-44 PMID: 4587405
  32. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation.
    J Mol Biol. 1968 Jan 28;31(2):291-304 PMID: 4865486
  33. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  34. A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):300-4 PMID: 319458
  35. Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation.
    Mol Gen Genet. 1976 Oct 18;148(2):125-30 PMID: 790151
  36. Ultraviolet-induced mutation and DNA repair.
    Annu Rev Microbiol. 1969;23:487-514 PMID: 4899079
  37. Effect of a recA gene on cell division and capsular polysaccharide production in a lon strain of Escherichia coli.
    Genet Res. 1969 Oct;14(2):159-62 PMID: 4904461
  38. Involvement of recA and exr genes in the in vivo inhibition of the recBC nuclease.
    J Bacteriol. 1974 May;118(2):465-70 PMID: 4597445
  39. Partial suppression of the LexA phenotype by mutations (rnm) which restore ultraviolet resistance but not ultraviolet mutability to Escherichia coli B/r uvr A lexA.
    Mutat Res. 1976 Jul;36(1):17-28 PMID: 781527
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283484
Published
1977-11-14
Pages
121-31
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283484
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2