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PMID: 340901 Published · ppublish English Journal Article

Analysis of heme biosynthesis in catalase and cytochrome deficient yeast mutants.

Molecular & general genetics : MGG ·Vol. 156 ·No. 2 ·1977-11-14 ·Pages 177-83

Labbe-Bois R, Rytka J, Litwinska J, Bilinski T

Abstract

Mutants of Saccharomyces cerevisiae, described as catalase and cytochromes deficient (Pachecka et al., 1974), have been analyzed for heme biosynthesis ability. Some enzymatic activities involved in protoheme synthesis were measured in acellular extracts, whereas whole cells were analyzed for cytochrome spectra and for possible accumulation of porphyrin synthesis intermediates. A good correlation was found between these in vitro and in vivo studies. Results show that two mutants were impaired in 5-aminolevulinate synthesis, two mutants were devoid of uroporphyrinogen I synthetase activity and one mutant presented defects in coproporphyrinogen III oxidase activity.

MeSH Terms
Catalase/metabolism Cytochromes/metabolism Heme/biosynthesis Mutation Phenotype Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Cytochromes Heme Catalase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Labbe-Bois R
Rytka J
Litwinska J
Bilinski T
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283490
Published
1977-11-14
Pages
177-83
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283490
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