Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid.

Molecular & general genetics : MGG ·Vol. 157 ·No. 1 ·1977-11-29 ·Pages 17-23

Lane D, Chandler M

Abstract

We have cloned the EcoRI fragments of pLC1, a circular DNA element found in an Escherichia coli dnaAts strain integratively suppressed by R100.1 (Chandler et al., 1977a), using the plasmid vector pCR1. All the resistance genes known to be present on the r-determinant of R100.1 were found to be present on pLC1. The isolation of pCR1 derivatives carrying various EcoRI fragments of either pLC1 or R100.1 has allowed a more precise mapping of the position of the resistance genes on the R100.1 molecule.

MeSH Terms

Chloramphenicol/pharmacology DNA, Bacterial DNA, Circular Escherichia coli/drug effects,genetics Fusidic Acid/pharmacology Genetic Linkage Mercury/pharmacology R Factors Streptomycin/pharmacology Sulfonamides/pharmacology Tetracycline/pharmacology

Chemicals

DNA, Bacterial DNA, Circular Sulfonamides Fusidic Acid Chloramphenicol Tetracycline Mercury Streptomycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lane D

Chandler M

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268682

Published

1977-11-29

Pages

17-23

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268682

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Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid.

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