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PMID: 340931 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro construction of plasmids which result in overproduction of the protein product of the araC gene of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 157 ·No. 3 ·1977-12-09 ·Pages 341-4

Steffen D, Schleif R

Abstract

Derivatives of the Escherichia coli drug resistance plasmid pMB-9 were constructed which contain the promoter from the lactose operon of E. coli fused to the araC gene of E. coli. E. coli possessing these plasmids contain about 50 times as much of the araC gene product as do cells with a wild-type araC gene and promotor.

MeSH Terms

Arabinose/biosynthesis,genetics Escherichia coli/genetics Genes Lactose Factors Plasmids Recombination, Genetic

Chemicals

Arabinose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Steffen D

Schleif R

References (12)

12 references, click to expand

Overproducing araC protein with lambda-arabinose transducing phage.
Mol Gen Genet. 1977 Dec 9;157(3):333-9 PMID: 340930
Amplification and characterization of a beta-globin gene synthesized in vitro.
Cell. 1976 Jun;8(2):163-82 PMID: 61066
Enzymatic end-to end joining of DNA molecules.
J Mol Biol. 1973 Aug 15;78(3):453-71 PMID: 4754844
Dual control of arabinose genes on transducing phage lambda-dara.
J Mol Biol. 1971 Jul 14;59(1):127-50 PMID: 4934313
Regulation of the regulatory gene for the arabinose pathway, araC.
J Mol Biol. 1976 Jul 5;104(3):557-66 PMID: 781294
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
Cell. 1974 Dec;3(4):315-25 PMID: 4216403
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
Nucleic Acids Res. 1976 Jan;3(1):101-16 PMID: 765970
An L-arabinose binding protein and arabinose permeation in Escherichia coli.
J Mol Biol. 1969 Nov 28;46(1):185-96 PMID: 4902208
Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H.
J Virol. 1976 May;18(2):659-63 PMID: 1083914
Different cyclic AMP requirements for induction of the arabinose and lactose operons of Escherichia coli.
J Mol Biol. 1973 Sep 5;79(1):149-62 PMID: 4355595
Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904-9 PMID: 4342968

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268672

Published

1977-12-09

Pages

341-4

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268672

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In vitro construction of plasmids which result in overproduction of the protein product of the araC gene of Escherichia coli.

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