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PMID: 3410498 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Platelet-activating factor induces eosinophil peroxidase release from purified human eosinophils.

Immunology ·Vol. 64 ·No. 3 ·1988-07-00 ·Pages 559-61

Kroegel C, Yukawa T, Dent G, Chanez P, Chung KF, Barnes PJ

Abstract

The degranulation response of purified human eosinophils to platelet-activating factor (PAF) has been studied. PAF induced release of eosinophil peroxidase (EPO) and beta-glucuronidase from highly purified human eosinophils with an EC50 of 0.9 nM. The order of release was comparable with that induced by phorbol myristate acetate (PMA). The new specific PAF antagonist 3-[4-(2-chlorophenyl)-9-methyl-H-thieno[3,2-f] [1,2,4]triazolo-[4,3a][1,4]-diazepin-2-yl](4-morpholinyl)- 1-propane-one (WEB 2086) inhibited the PAF-induced enzyme release by human eosinophils in a dose-dependent manner. The viability of eosinophils were unaffected both by PAF and WEB 2086. The results suggest that PAF may amplify allergic and inflammatory reactions by release of preformed proteins from eosinophil granules.

MeSH Terms
Azepines/pharmacology Eosinophil Peroxidase Eosinophils/enzymology Humans Peroxidases/metabolism Platelet Activating Factor/antagonists & inhibitors,physiology Triazines/pharmacology Triazoles
Chemicals
Azepines Platelet Activating Factor Triazines Triazoles WEB 2086 Eosinophil Peroxidase Peroxidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kroegel C
Department of Thoracic Medicine, Brompton Hospital, London.
Yukawa T
Dent G
Chanez P
Chung K F
Barnes P J
References (19)
19 references, click to expand
  1. A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni.
    J Immunol. 1979 Apr;122(4):1228-36 PMID: 448088
  2. Lactic dehydrogenase activity in blood.
    Proc Soc Exp Biol Med. 1955 Oct;90(1):210-3 PMID: 13273400
  3. The interaction of human eosinophils and neutrophils with non-phagocytosable surfaces: a model for studying cell-mediated immunity in schistosomiasis.
    Parasitology. 1980 Jun;80(3):525-37 PMID: 7393621
  4. Eosinophil peroxidase-induced mast cell secretion.
    J Exp Med. 1980 Aug 1;152(2):265-79 PMID: 6156983
  5. Generation of the eosinophil chemotactic factor (ECF) from various cell types by melittin.
    Mol Immunol. 1981 Mar;18(3):227-36 PMID: 7266488
  6. A simple reliable assay for myeloperoxidase activity in mixed neutrophil-eosinophil cell suspensions: application to detection of myeloperoxidase deficiency.
    J Immunol Methods. 1984 May 11;70(1):119-25 PMID: 6325545
  7. Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density.
    Clin Exp Immunol. 1984 Sep;57(3):735-42 PMID: 6467687
  8. Human eosinophil peroxidase: purification and characterization.
    J Immunol. 1985 Mar;134(3):1875-9 PMID: 3918110
  9. Extracellular release of rat eosinophil peroxidase (EPO) I. Role of anaphylactic immunoglobulins.
    J Immunol. 1985 Mar;134(3):1968-74 PMID: 3968436
  10. Calcium ionophore A23187 calcium-dependent cytolytic degranulation in human eosinophils.
    J Immunol. 1985 Aug;135(2):1349-56 PMID: 3925008
  11. Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors.
    J Immunol Methods. 1985 Nov 7;83(2):209-15 PMID: 3840509
  12. The role of the eosinophilic leukocyte in bronchial asthma.
    Bull Eur Physiopathol Respir. 1986;22 Suppl 7:62-9 PMID: 3955268
  13. Role of specific IgE antibodies in peroxidase (EPO) release from human eosinophils.
    J Immunol. 1986 Sep 1;137(5):1659-64 PMID: 3489038
  14. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils.
    J Clin Invest. 1986 Dec;78(6):1701-6 PMID: 3023451
  15. The eosinophilic leukocyte: structure and function.
    Adv Immunol. 1986;39:177-253 PMID: 3538819
  16. The injurious effect of eosinophil peroxidase, hydrogen peroxide, and halides on pneumocytes in vitro.
    J Allergy Clin Immunol. 1987 Mar;79(3):496-504 PMID: 3029204
  17. Pharmacological actions of WEB 2086, a new specific antagonist of platelet activating factor.
    J Pharmacol Exp Ther. 1987 Jun;241(3):974-81 PMID: 3598913
  18. Beta-glucuronidase.
    Adv Enzymol Relat Subj Biochem. 1955;16:361-409 PMID: 14376216
  19. Eosinophil-mediated mammalian tumor cell cytotoxicity: role of the peroxidase system.
    J Immunol. 1980 Apr;124(4):1949-53 PMID: 7365245
Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1988-07-00
Pages
559-61
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1385073
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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