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PMID: 341154 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of the silk fibroin gene and its flanking sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5363-7

Ohshima Y, Suzuki Y

Abstract

Thirteen Escherichia coli clones containing the whole or a part of the fibroin gene (16 kilobases long) have been isolated. The starting material was DNA extracted from the posterior silk glands of Bombyx mori. Most clones were obtained from sheared DNA fragments linked by poly(dA)-poly(dT) joints to the plasmid pMB9. One of them includes the 5' end of the fibroin gene with a flanking sequence of 12 kilobases, and another includes the 3' end of the gene with a flanking sequence of about 1 kilobase. One clone was obtained by ligation, to pMB9, of a fragment generated by endodeoxy-ribonuclease EcoRI. This clone has a 21-kilobase insertion that probably includes the entire fibroin gene with flanking sequences at both ends. The cleavage sites for endodeoxyribonucleases EcoRI, HindIII, and BamHI have been established for the cloned sequences.

MeSH Terms
Base Sequence Bombyx/genetics Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Recombinant/isolation & purification Escherichia coli/genetics Fibroins/genetics Genes Plasmids
Chemicals
DNA, Recombinant Fibroins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohshima Y
Suzuki Y
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5363
Published
1977-12-00
Pages
5363-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431721
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5363 PMC Full Text View journal details
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