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PMID: 3412894 Published · ppublish English Journal Article

Multiple nuclear factors interact with promoter sequences of the urokinase-type plasminogen activator gene.

Nucleic acids research ·Vol. 16 ·No. 15 ·1988-08-11 ·Pages 7527-44

von der Ahe D, Pearson D, Nakagawa J, Rajput B, Nagamine Y

Abstract

To characterize proteins that bind to the cyclic AMP inducible promoter of the urokinase-type plasminogen activator gene, we performed a DNAase I footprinting analysis. Within 500 nucleotides upstream of the transcription start site we found eight protected regions due to at least four different binding proteins. Among these is a single binding site for the transcription factor CTF/NF1, which is flanked on each side by two conserved binding sites for the transcription factor Sp1. A region at -380, which shares a similarity with sequences observed in the corresponding regions of other cyclic AMP regulated genes, was protected. This binding site contains a sequence of ten nucleotides which is repeated further upstream at -480 and also protected against DNAase I digestion. Comparisons of extracts from four different cell lines revealed that all DNA binding factors are present in nuclei of uPA expressing and nonexpressing cells. Mechanism underlying hormonal regulation of the gene is discussed.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA-Binding Proteins/physiology Deoxyribonuclease I Molecular Sequence Data Molecular Weight Nuclear Proteins/physiology Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Swine Transcription Factors/physiology Urokinase-Type Plasminogen Activator/genetics
Chemicals
DNA-Binding Proteins Nuclear Proteins Transcription Factors Deoxyribonuclease I Urokinase-Type Plasminogen Activator
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
von der Ahe D
Friedrich Miescher-Institut, Basel, Switzerland.
Pearson D
Nakagawa J
Rajput B
Nagamine Y
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.15.7527
Published
1988-08-11
Pages
7527-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338425
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.15.7527 PMC Full Text View journal details
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