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PMID: 3419517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Amplification and analysis of DNA sequences in single human sperm and diploid cells.

Nature ·Vol. 335 ·No. 6189 ·1988-09-29 ·Pages 414-7

Li HH, Gyllensten UB, Cui XF, Saiki RK, Erlich HA, Arnheim N

Abstract

The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.

MeSH Terms
Base Sequence Cell Line Chromosome Mapping Chromosomes DNA DNA-Directed DNA Polymerase Gene Amplification Globins/genetics HLA-DQ Antigens/genetics Humans Male Nucleic Acid Hybridization Receptors, LDL/genetics Recombination, Genetic Spermatozoa/ultrastructure
Chemicals
HLA-DQ Antigens Receptors, LDL Globins DNA DNA-Directed DNA Polymerase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Li H H
Department of Biological Sciences, University of Southern California, Los Angeles 90089-0371.
Gyllensten U B
Cui X F
Saiki R K
Erlich H A
Arnheim N
Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/335414a0
Published
1988-09-29
Pages
414-7
Language
English
Region
England
NLM ID
0410462
Subset
IM
External Links
PubMed source DOI: 10.1038/335414a0 View journal details
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