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PMID: 3422101 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Location and characterization of two widely separated glucocorticoid response elements in the phosphoenolpyruvate carboxykinase gene.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 96-104

Petersen DD, Magnuson MA, Granner DK

Abstract

Chimeric genes were constructed by fusion of various regions of the 5'-flanking sequence from the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene to the chloramphenicol acetyltransferase-coding sequence and to simian virus 40 splice and polyadenylation sequences. These were used to demonstrate that two glucocorticoid regulatory elements (GREs) combine to confer glucocorticoid responsiveness upon the PEPCK gene in H4IIE hepatoma cells. Both elements, a distal one whose 5' boundary is located between -1264 and -1111 base pairs and a proximal one located between -468 and -420 base pairs relative to the transcription initiation site, act independently, in various positions and orientations, and upon the heterologous thymidine kinase promoter. Each element accounts for half of the maximal response of the chimeric genes. Therefore, two widely separated enhancerlike elements contribute equally to the response of the PEPCK gene to glucocorticoid hormones. Neither of the PEPCK GREs contains the TGTTCT consensus sequence associated with most other GREs.

MeSH Terms
Acetyltransferases/genetics Animals Chloramphenicol O-Acetyltransferase Chromosome Mapping DNA Mutational Analysis Gene Expression Regulation Glucocorticoids/physiology Phosphoenolpyruvate Carboxykinase (GTP)/genetics Promoter Regions, Genetic Rats Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Glucocorticoids Recombinant Fusion Proteins Acetyltransferases Chloramphenicol O-Acetyltransferase Phosphoenolpyruvate Carboxykinase (GTP)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Petersen D D
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232.
Magnuson M A
Granner D K
References (40)
40 references, click to expand
  1. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  2. Multihormonal regulation of phosphoenolpyruvate carboxykinase-chloramphenicol acetyltransferase fusion genes. Insulin's effects oppose those of cAMP and dexamethasone.
    J Biol Chem. 1987 Nov 5;262(31):14917-20 PMID: 2822706
  3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  4. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element.
    Cell. 1983 Jul;33(3):695-703 PMID: 6871991
  5. Inhibition of transcription of the phosphoenolpyruvate carboxykinase gene by insulin.
    Nature. 1983 Oct 6-12;305(5934):549-51 PMID: 6353247
  6. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
    Cell. 1983 Oct;34(3):865-79 PMID: 6313211
  7. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.
    Gene. 1981 Mar;13(2):197-202 PMID: 6263759
  10. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
    Nature. 1981 Nov 19;294(5838):228-32 PMID: 6272123
  11. N6,O2'-dibutyryl cycle AMP and glucose regulate the amount of messenger RNA coding for hepatic phosphoenolpyruvate carboxykinase (GTP).
    J Biol Chem. 1982 Feb 25;257(4):2022-8 PMID: 6276405
  12. Purified glucocorticoid receptor-hormone complex from rat liver cytosol binds specifically to cloned mouse mammary tumor virus long terminal repeats in vitro.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5157-61 PMID: 6291027
  13. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  14. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo.
    Cell. 1983 Jun;33(2):489-99 PMID: 6190571
  15. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  16. Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region.
    Cell. 1983 Dec;35(2 Pt 1):381-92 PMID: 6317184
  17. Activation of a heterologous promoter in response to dexamethasone and cadmium by metallothionein gene 5'-flanking DNA.
    Cell. 1984 Feb;36(2):371-9 PMID: 6692472
  18. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  19. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  20. Multihormonal regulation of phosphoenolpyruvate carboxykinase gene transcription. The dominant role of insulin.
    J Biol Chem. 1984 Dec 25;259(24):15242-51 PMID: 6096365
  21. Steroid hormone regulation of gene expression.
    Annu Rev Pharmacol Toxicol. 1985;25:529-66 PMID: 2988423
  22. Rat hepatic cytosolic phosphoenolpyruvate carboxykinase (GTP). Structures of the protein, messenger RNA, and gene.
    J Biol Chem. 1985 Sep 5;260(19):10748-60 PMID: 2993287
  23. Upstream promoter element of the human metallothionein-IIA gene can act like an enhancer element.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8572-6 PMID: 3866241
  24. Steroid receptor regulated transcription of specific genes and gene networks.
    Annu Rev Genet. 1985;19:209-52 PMID: 3909942
  25. A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):710-4 PMID: 3003746
  26. In vivo competition between a metallothionein regulatory element and the SV40 enhancer.
    Science. 1986 Apr 4;232(4746):76-80 PMID: 3006253
  27. The SV40 enhancer is composed of multiple functional elements that can compensate for one another.
    Cell. 1986 May 9;45(3):461-70 PMID: 3009027
  28. Mechanism of gene regulation by steroid hormones.
    J Steroid Biochem. 1986 Jan;24(1):19-24 PMID: 3009974
  29. Glucocorticoid responsiveness of the transcriptional enhancer of Moloney murine sarcoma virus.
    Cell. 1986 Jul 18;46(2):283-90 PMID: 3013424
  30. Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. I. Multiple hormone regulatory elements and the effects of enhancers.
    J Biol Chem. 1986 Jul 25;261(21):9714-20 PMID: 3015902
  31. Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains.
    J Biol Chem. 1986 Jul 25;261(21):9721-6 PMID: 3015903
  32. Glucocorticoid receptor binding and activation of a heterologous promoter by dexamethasone by the first intron of the human growth hormone gene.
    Mol Cell Biol. 1985 Nov;5(11):2984-92 PMID: 3018491
  33. Gene regulation by proteins acting nearby and at a distance.
    Nature. 1986 Aug 21-27;322(6081):697-701 PMID: 3018583
  34. Functional analysis of the glucocorticoid regulatory elements present in the mouse mammary tumor virus long terminal repeat. A synthetic distal binding site can replace the proximal binding domain.
    J Mol Biol. 1986 Aug 5;190(3):367-78 PMID: 3023640
  35. Two different factors act separately or together to specify functionally distinct activities at a single transcriptional enhancer.
    Mol Cell Biol. 1986 Apr;6(4):993-1001 PMID: 3023887
  36. Glucocorticoid-regulated expression from the 5'-flanking region of the rat alpha 1-acid glycoprotein gene. Requirement for ongoing protein synthesis.
    J Biol Chem. 1987 Jan 15;262(2):520-3 PMID: 3804995
  37. Multihormonal regulation of transcription of the tryptophan 2,3-dioxygenase gene in primary cultures of adult rat hepatocytes with special reference to the presence of a transcriptional protein mediating the action of glucocorticoids.
    J Biol Chem. 1987 Jan 15;262(2):727-33 PMID: 3542992
  38. Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene.
    Cell. 1987 Apr 10;49(1):29-38 PMID: 2881624
  39. Glucocorticoid induction of the rat tryptophan oxygenase gene is mediated by two widely separated glucocorticoid-responsive elements.
    EMBO J. 1987 Mar;6(3):625-30 PMID: 3582368
  40. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.96-104.1988
Published
1988-01-00
Pages
96-104
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363086
Subset
IM
Grants
NIADDK NIH HHS · 5T32AM07061-13 · United States
NIDDK NIH HHS · DK07061 · United States
NIDDK NIH HHS · DK35107 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.1.96-104.1988 PMC Full Text View journal details
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