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PMID: 3429620 Published · ppublish English Journal Article

Specificity of a Mycobacterium kansasii phenolic glycolipid (mycoside A) immunoserum.

Journal of clinical microbiology ·Vol. 25 ·No. 12 ·1987-12-00 ·Pages 2270-3

Papa F, Riviere M, Fournié JJ, Puzo G, David H

Abstract

The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii showed that all strains of M. kansasii and the representative strain of M. gastri formed the antigen, whereas none of the remaining species (including M. leprae) formed it. Consequently, it was found that the antiserum was highly specific and useful for diagnostic purposes.

MeSH Terms
Animals Antibodies, Bacterial/immunology Antibody Specificity Chromatography, Thin Layer Enzyme-Linked Immunosorbent Assay Glycolipids/immunology Immune Sera/immunology Mycobacterium/immunology Nontuberculous Mycobacteria/immunology Rabbits Species Specificity
Chemicals
Antibodies, Bacterial Glycolipids Immune Sera mycoside A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Papa F
Service de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
Riviere M
Fournié J J
Puzo G
David H
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.25.12.2270-2273.1987
Published
1987-12-00
Pages
2270-3
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC269468
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.25.12.2270-2273.1987 PMC Full Text View journal details
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