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PMID: 3431546 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Functional analysis and growth factor regulation of the human vimentin promoter.

Molecular and cellular biology ·Vol. 7 ·No. 11 ·1987-11-00 ·Pages 3908-15

Rittling SR, Baserga R

Abstract

Vimentin is a growth-regulated gene whose mRNA levels increase severalfold after stimulation of quiescent cells. We have isolated and sequenced a genomic fragment of human DNA containing the vimentin 5'-flanking sequence and untranslated region. S1 nuclease analysis was used to determine the transcription initiation site. Deletion mutants of the promoter region were constructed, linked to a chloramphenicol acetyltransferase gene, and analyzed for transient expression by transfection into BALB/c 3T3 cells. These experiments revealed the presence in the human vimentin promoter region of a negative-regulatory element, flanked by positive elements. The most 5' of the positive elements is able to overcome the effects of the negative element. Analysis of these deletion constructs in stable cell lines confirmed the results of the transient assays. Using these stable cell lines, we can also demonstrate that the vimentin promoter region can confer platelet-derived growth factor inducibility to a linked chloramphenicol acetyltransferase gene and that the sequences required for this inducibility reside between positions -241 and +73.

MeSH Terms
Animals Base Sequence Cells, Cultured Chromosome Deletion Cloning, Molecular DNA/metabolism Genes Genes, Regulator/drug effects Humans Liver/metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Platelet-Derived Growth Factor/pharmacology Promoter Regions, Genetic RNA, Messenger/genetics Transcription, Genetic Vimentin/genetics
Chemicals
Platelet-Derived Growth Factor RNA, Messenger Vimentin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rittling S R
Department of Pathology, Temple University Medical School, Philadelphia, Pennsylvania 19140.
Baserga R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.11.3908-3915.1987
Published
1987-11-00
Pages
3908-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368058
Subset
IM
Grants
NIA NIH HHS · AG-00378 · United States
Databases
GENBANK
M17888
External Links
PubMed source DOI: 10.1128/mcb.7.11.3908-3915.1987 PMC Full Text View journal details
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