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PMID: 3456603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of the binding site on 5S rRNA for the transcription factor IIIA: proposed structure of a common binding site on 5S rRNA and on the gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 6 ·1986-03-00 ·Pages 1593-7

Huber PW, Wool IG

Abstract

Transcription factor IIIA interacts specifically with an internal control region of Xenopus 5S ribosomal RNA genes and is also a component, along with 5S rRNA, of a 7S ribonucleoprotein particle present in previtellogenic oocytes. We have determined the region of the 5S rRNA in the 7S ribonucleoprotein complex that is protected by the transcription factor from digestion with the ribonuclease alpha-sarcin. The binding site for factor IIIA extends from nucleotide 64 through nucleotide 116; the protected region includes two CCUGG helices separated by 11 nucleotides. The same helices occur in the factor IIIA binding site in the 5S rRNA gene and may constitute a common structural feature recognized by the protein in the gene and in the gene product.

MeSH Terms
Animals Base Sequence Binding Sites Endoribonucleases Fungal Proteins/metabolism Genes Nucleic Acid Conformation Oligonucleotides/analysis Oocytes/analysis RNA, Ribosomal/metabolism Transcription Factor TFIIIA Transcription Factors/metabolism Xenopus laevis/metabolism
Chemicals
Fungal Proteins Oligonucleotides RNA, Ribosomal Transcription Factor TFIIIA Transcription Factors alpha-sarcin Endoribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huber P W
Wool I G
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.6.1593
Published
1986-03-00
Pages
1593-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323129
Subset
IM
Grants
NIGMS NIH HHS · GM33702 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.6.1593 PMC Full Text View journal details
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