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PMID: 3457367 Published · ppublish English Journal Article

The role of the loxP spacer region in P1 site-specific recombination.

Nucleic acids research ·Vol. 14 ·No. 5 ·1986-03-11 ·Pages 2287-300

Hoess RH, Wierzbicki A, Abremski K

Abstract

The lox-Cre site-specific recombination system of bacteriophage P1 is comprised of a site on the DNA where recombination occurs called loxP, and a protein, Cre, which mediates the reaction. The loxP site is 34 base pairs (bp) in length and consists of two 13 bp inverted repeats separated by an 8 bp spacer region. Previously it has been shown that the cleavage and strand exchange of recombining loxP sites occurs within this spacer region. We report here an analysis of various base substitution mutations within the spacer region of loxP, and conclude the following: Homology is a requirement for efficient recombination between recombining loxP sites. There is at least one position within the spacer where a base change drastically reduces recombination even when there is homology between the two recombining loxP sites. When two loxP sites containing symmetric spacer regions undergo Cre-mediated recombination in vitro, the DNA between the sites undergoes both excision and inversion with equal frequency.

MeSH Terms
Bacterial Proteins/genetics Bacteriophages/genetics Base Sequence Cloning, Molecular DNA, Superhelical/genetics DNA, Viral/genetics DNA-Binding Proteins/genetics Genetic Engineering Recombination, Genetic
Chemicals
Bacterial Proteins DNA, Superhelical DNA, Viral DNA-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoess R H
Wierzbicki A
Abremski K
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.5.2287
Published
1986-03-11
Pages
2287-300
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339658
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.5.2287 PMC Full Text View journal details
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