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PMID: 346483 Published · ppublish English Journal Article

Isoelectric point of cell-free K99 antigen exhibiting hemagglutinating properties.

Infection and immunity ·Vol. 19 ·No. 3 ·1978-03-00 ·Pages 1097-8

Morris JA, Stevens AE, Sojka WJ

Abstract

The isoelectric point of the K99 antigen in partially purified preparations isolated from Escherichia coli B41 was 4.2. Electrofocused K99 antigen hemagglutinated guinea pig and sheep erythrocytes and gave a single precipitin line on diffusion against antisera to E. coli B41 and absorbed factor K99 antisera.

MeSH Terms
Agglutinins Antigens, Bacterial Escherichia coli/immunology Hemagglutinins Isoelectric Point Plasmids
Chemicals
Agglutinins Antigens, Bacterial Hemagglutinins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morris J A
Stevens A E
Sojka W J
References (7)
7 references, click to expand
  1. K99 surface antigen of Escherichia coli: purification and partial characterization.
    Infect Immun. 1977 Jan;15(1):272-9 PMID: 401772
  2. Preliminary characterization of cell-free K99 antigen isolated from Escherichia coli B41.
    J Gen Microbiol. 1977 Apr;99(2):353-7 PMID: 327023
  3. Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli.
    J Gen Microbiol. 1976 Oct;96(2):269-75 PMID: 792386
  4. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains.
    J Med Microbiol. 1972 May;5(2):243-50 PMID: 4555733
  5. Etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and K99 antigen production by Escherichia cola.
    Am J Vet Res. 1976 Sep;37(9):1025-9 PMID: 786085
  6. The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains.
    Acta Pathol Microbiol Scand B. 1975 Feb;83(1):31-6 PMID: 1093354
  7. Occurrence and characteristics of enterotoxigenic Escherichia coli isolated from calves with diarrhea.
    Infect Immun. 1976 Apr;13(4):1117-9 PMID: 776828
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
1978-03-00
Pages
1097-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC422302
Subset
IM
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