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PMID: 346567 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

Journal of bacteriology ·Vol. 133 ·No. 3 ·1978-03-00 ·Pages 1287-94

Donoghue DJ, Sharp PA

Abstract

A derivative of bacteriophage lambda containing a colicin E1 plasmid replicon was constructed by recombinant DNA techniques. This phage, lambdacol100, has two functional modes of DNA replication; it can replicate via either plasmid or phage replication systems. lambdacol100 has been used to introduce the colicin E1 plasmid replicon into Escherichia coli previously treated with chloramphenicol to block protein synthesis. Under these conditions, lambdacol100 DNA is replicated normally as a colicin E1 plasmid. This suggests that colicin E1 plasmid replication in vivo does not require any plasmid-encoded proteins.

MeSH Terms
Bacterial Proteins/biosynthesis Chloramphenicol/pharmacology Colicins/biosynthesis Coliphages/genetics DNA Replication DNA, Recombinant Escherichia coli/genetics,metabolism Plasmids
Chemicals
Bacterial Proteins Colicins DNA, Recombinant Chloramphenicol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Donoghue D J
Sharp P A
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.3.1287-1294.1978
Published
1978-03-00
Pages
1287-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222164
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.3.1287-1294.1978 PMC Full Text View journal details
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