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PMID: 3466181 Published · ppublish English Journal Article

A synthetic peptide vaccine involving the product of the pre-S(2) region of hepatitis B virus DNA: protective efficacy in chimpanzees.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 23 ·1986-12-00 ·Pages 9174-8

Itoh Y, Takai E, Ohnuma H, Kitajima K, Tsuda F, Machida A, Mishiro S, Nakamura T, Miyakawa Y, Mayumi M

Abstract

The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the sequence of 19 amino acid residues (amino acids 14-32 from the N terminus) representing an area of high local hydrophilicity is shared by viral strains of subtypes adr, ayw, and ayr; residue 22, phenylalanine, is replaced by leucine in a strain of the other subtype, adw. A synthetic peptide vaccine involving these 19 amino acid residues, when given to two chimpanzees, raised antibodies that bound to viral particles and protected the animals from challenge with 10(6) chimpanzee infectious doses of hepatitis B virus.

MeSH Terms
Animals Antigens/immunology Genes, Viral Hepatitis B/prevention & control Hepatitis B Surface Antigens/immunology Hepatitis B virus/classification,genetics,immunology Oligopeptides/immunology Pan troglodytes Species Specificity Vaccines, Synthetic/immunology Viral Proteins/immunology Viral Vaccines/immunology
Chemicals
Antigens Hepatitis B Surface Antigens Oligopeptides Vaccines, Synthetic Viral Proteins Viral Vaccines
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Itoh Y
Takai E
Ohnuma H
Kitajima K
Tsuda F
Machida A
Mishiro S
Nakamura T
Miyakawa Y
Mayumi M
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.23.9174
Published
1986-12-00
Pages
9174-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387097
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.23.9174 PMC Full Text View journal details
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