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PMID: 3472209 Published · ppublish English Journal Article

Chemical carcinogen-induced decreases in genomic 5-methyldeoxycytidine content of normal human bronchial epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 10 ·1987-05-00 ·Pages 3298-301

Wilson VL, Smith RA, Longoria J, Liotta MA, Harper CM, Harris CC

Abstract

The genomic content of DNA 5-methyldeoxycytidine (m5dC) was measured in dividing normal human bronchial epithelial cells treated with a broad range of chemical carcinogens. At noncytotoxic concentrations, all of the carcinogenic agents tested significantly reduced cellular DNA m5dC content whereas the weakly carcinogenic and noncarcinogenic agents, benzo[e]pyrene and phenanthrene (respectively), did not. These reductions varied from 8% to 31% depending on the agent and the donor cells. The reductions in genomic m5dC levels were concentration dependent for the carcinogenic polycyclic aromatic hydrocarbon benzo[a]pyrene. We speculate that carcinogen-induced perturbation of DNA m5dC patterns may lead to heritable changes in gene expression and contribute to the molecular alterations involved in the initiation and the subsequent steps of the carcinogenesis process.

MeSH Terms
Carcinogens/pharmacology Cells, Cultured DNA/drug effects Deoxycytidine/analogs & derivatives,metabolism Epithelial Cells Humans Lung/cytology,drug effects
Chemicals
Carcinogens Deoxycytidine DNA 5-methyldeoxycytidine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wilson V L
Smith R A
Longoria J
Liotta M A
Harper C M
Harris C C
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.10.3298
Published
1987-05-00
Pages
3298-301
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304856
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.10.3298 PMC Full Text View journal details
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