Cloning of a restriction fragment of phage mu DNA coding for early functions.

Molecular & general genetics : MGG ·Vol. 160 ·No. 1 ·1978-03-20 ·Pages 115-8

Schumann W, Bade EG, Delius H, Hübert P

Abstract

The DNA of an E. coli K12 strain harboring ten wildtype Mu prophages was restricted with endonuclease EcoRI, and the fragments ligated into the plasmid vector pMB9. Upon transformation of a strain carrying a heat inducible (Mu cts62) prophage, one temperature-resistant transformant was isolated. This transformant strain harbors the hybrid plasmid pKN001, containing the EcoRI.C fragment of Mu DNA as shown by restriction and heteroduplex analysis. Stable transformants of pKN001 are immune to superinfection with phage Mu. Transformation of Mu sensitive bacteria with pKN001 results in killing of the recipients (10(-4) surviving bacteria). The killing function is not expressed upon transformation of Mu-immune (lysogenic) bacteria.

MeSH Terms

Cell Transformation, Viral Coliphages/genetics DNA, Bacterial/genetics DNA, Recombinant/genetics DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Plasmids

Chemicals

DNA, Bacterial DNA, Recombinant DNA, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schumann W

Bade E G

Delius H

Hübert P

References (9)

9 references, click to expand

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Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.
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Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.
Nature. 1973 Jan 12;241(5385):120-3 PMID: 4572006
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
Molecular biology of bacteriophage mu.
Science. 1975 Nov 14;190(4215):624-32 PMID: 1103291
Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904-9 PMID: 4342968
Participation of Escherichia coli DNA in the replication of temperate bacteriophage Mu1.
Virology. 1974 Aug;60(2):534-42 PMID: 4602346

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00275127

Published

1978-03-20

Pages

115-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00275127

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Cloning of a restriction fragment of phage mu DNA coding for early functions.

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