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PMID: 347395 Published · ppublish English Comparative Study Journal Article

The nucleoside sequence of tyrosine tRNA from Bacillus stearothermophilus.

Nucleic acids research ·Vol. 5 ·No. 1 ·1978-01-00 ·Pages 23-36

Brown RS, Rubin JR, Rhodes D, Guilley H, Simoncsits A, Brownlee GG

Abstract

The nucleotide sequence of tRNATyr from B. stearothermophilus has been determined: pG-G-A-G-G-G-G-s4U-A-G-C-G-A-A-G-U-Gm-G-C-U-A-A-m1A-C-G-C-G-G-C-G-G-A-C-U-Q-U-A-ms2i6A-A-psi-C-C-G-C-U-C-C-C-U-U-U-G-G-G-U-U-C-G-G-C-G-G-T-psi-C-G-A-A-U-C-C-G-U-C-C-C-C-C-U-C-C-A-C-C-AOH. A combination of classical fingerprinting methods, partial nuclease P1 digestion and two-dimensional homochromatography and a rapid "read off" sequencing gel technique were used to establish the complete nucleotide sequence.

MeSH Terms
Base Sequence Escherichia coli Geobacillus stearothermophilus/analysis RNA, Bacterial/metabolism RNA, Transfer/metabolism Species Specificity Tyrosine Tyrosine-tRNA Ligase/metabolism
Chemicals
RNA, Bacterial Tyrosine RNA, Transfer Tyrosine-tRNA Ligase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brown R S
Rubin J R
Rhodes D
Guilley H
Simoncsits A
Brownlee G G
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1978-01-00
Pages
23-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341959
Subset
IM
Databases
GENBANK
K00270
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