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PMID: 347444 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Positive control of D-serine deaminase synthesis in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 4 ·1978-04-00 ·Pages 1695-9

Heincz MC, Kelker NE, McFall E

Abstract

Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA. Synthesis from a dsdO+ dsdA+ template requires active dsdC gene product and 3':5'-cyclic AMP. In vitro synthesis of dsdC product is obtained with a dsdC+ dsdO+ dsdA+ or a dsdCc dsdO+ dsdA+ template; this synthesis is thermosensitive and can be uncoupled from D-serine deaminase synthesis by temperature shift.

MeSH Terms

Cell-Free System Coliphages/genetics Enzyme Induction Escherichia coli/enzymology,genetics Genes Genes, Regulator L-Serine Dehydratase/biosynthesis,genetics Operon Serine/pharmacology Templates, Genetic

Chemicals

Serine L-Serine Dehydratase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Heincz M C

Kelker N E

McFall E

References (13)

13 references, click to expand

PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
J Biol Chem. 1965 Jun;240:2468-77 PMID: 14304855
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Regulation of argA operon expression in Escherichia coli K-12: cell-free synthesis of beta-galactosidase under argA control.
J Bacteriol. 1977 Oct;132(1):67-72 PMID: 410786
Construction of plasmids carrying the cI gene of bacteriophage lambda.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
Specialized transduction of D-serine deaminase genes: formation of lysogens that yield high lambda-d dsd/lambda ratios and formation of a dimeric lambda-d dsd.
J Bacteriol. 1976 Aug;127(2):998-1014 PMID: 783126
In vitro synthesis of protein in microbial systems.
Annu Rev Genet. 1973;7:267-87 PMID: 4593305
Role of adenosine 3',5'-cyclic monophosphate and its specific binding protein in the regulation of D-serine deaminase synthesis.
J Bacteriol. 1973 Feb;113(2):781-5 PMID: 4347927
D-serine dehydratase from Escherichia coli. II. Analytical studies and subunit structure.
J Biol Chem. 1970 Sep 25;245(18):4618-28 PMID: 4917239
A possible termination factor for transcription in Escherichia coli.
Biochem Biophys Res Commun. 1974 Feb 4;56(3):725-31 PMID: 4363749
Escape synthesis of D-serine deaminase in lambda dsdC integration lysogens.
J Bacteriol. 1976 Aug;127(2):1028-9 PMID: 134024
Positive control in the D-serine deaminase system of Escherichia coli K-12.
J Bacteriol. 1975 Mar;121(3):1092-101 PMID: 1090589
Escherichia coli K-12 mutant forming a temperature-sensitive D-serine deaminase.
J Bacteriol. 1975 Mar;121(3):1074-7 PMID: 1090587
Purification and properties of D-serine dehydrase from Escherichia coli.
J Biol Chem. 1966 Mar 10;241(5):1233-8 PMID: 5327100

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.4.1695

Published

1978-04-00

Pages

1695-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392405

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.4.1695 PMC Full Text View journal details

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Positive control of D-serine deaminase synthesis in vitro.

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