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PMID: 3475713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 16 ·1987-08-00 ·Pages 5962-6

Lin CR, Kapiloff MS, Durgerian S, Tatemoto K, Russo AF, Hanson P, Schulman H, Rosenfeld MG

Abstract

A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain Chemistry Calcium/metabolism Calmodulin/metabolism Cloning, Molecular DNA/analysis Gene Expression Regulation Isoenzymes/genetics Protein Kinases/genetics RNA, Messenger/metabolism Rats
Chemicals
Calmodulin Isoenzymes RNA, Messenger DNA Protein Kinases Calcium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lin C R
Kapiloff M S
Durgerian S
Tatemoto K
Russo A F
Hanson P
Schulman H
Rosenfeld M G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.16.5962
Published
1987-08-00
Pages
5962-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298983
Subset
IM
Databases
GENBANK
J02942
External Links
PubMed source DOI: 10.1073/pnas.84.16.5962 PMC Full Text View journal details
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