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PMID: 3485798 Published · ppublish English Comparative Study Journal Article

Two distinct T-cell receptor alpha-chain transcripts in a rabbit T-cell line: implications for allelic exclusion in T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 7 ·1986-04-00 ·Pages 2190-4

Marche PN, Kindt TJ

Abstract

Information relevant to allelic exclusion in T cells has been obtained by a study of cDNA clones corresponding to alpha-chain genes of the T-cell receptor in the rabbit T-cell line RL-5. One clone contains a variable-joining-constant (VJC) sequence encoding a complete alpha chain of the T-cell receptor. A second has an identical constant region and includes a distinct variable-joining (VJ) sequence. However, a single-base deletion in the variable region places the remainder of the second transcript out-of-phase and appears to be the product of a rearrangement involving a variable region of the T-cell receptor alpha-chain pseudogene. Presence of two variable-joining-constant (VJC) transcripts in the same cell line indicates that alpha-chain gene rearrangement is not affected by transcription of a complete alpha-chain mRNA and suggests that steps after mRNA synthesis are involved in the allelic exclusion process for alpha-chain genes. Comparison of rabbit alpha-chain sequences with those of man and mouse revealed interspecies conservation in constant and variable regions. Genomic Southern blot analyses using a rabbit constant region of the T-cell receptor alpha-chain probe revealed the presence of a single constant region gene. Hybridization with variable region probes defined two distinct multigenic subfamilies. Homology between certain rabbit and murine variable regions of the T-cell receptor alpha-chain sequences suggests that the existence of subfamilies predated divergence of these species.

MeSH Terms
Alleles Animals Biological Evolution Cell Line Cloning, Molecular DNA/genetics Gene Expression Regulation Humans Mice Peptide Chain Initiation, Translational RNA, Messenger/genetics Rabbits Receptors, Antigen, T-Cell/genetics T-Lymphocytes/physiology Transcription, Genetic
Chemicals
RNA, Messenger Receptors, Antigen, T-Cell DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Marche P N
Kindt T J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.7.2190
Published
1986-04-00
Pages
2190-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323257
Subset
IM
Databases
GENBANK
M12735, M12736, M12884, M12885
External Links
PubMed source DOI: 10.1073/pnas.83.7.2190 PMC Full Text View journal details
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