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PMID: 348679 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Polyamine auxotrophs of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 134 ·No. 1 ·1978-04-00 ·Pages 214-20

Whitney PA, Morris DR

Abstract

Strains of yeast have been constructed that are unable to synthesize ornithine and are thereby deficient in polyamine biosynthesis. These strains were used to develop a protocol for isolation of mutants blocked directly in polyamine synthesis. There were seven mutants isolated that lack ornithine decarboxylase activity; these strains exhibited greatly decreased pool levels of putrescine, spermidine, and spermine when grown in the absence of polyamines. Three of the mutants lack S-adenosylmethionine decarboxylase activity; polyamine limitation of a representative mutant resulted in an accumulation of putrescine and a decrease in spermidine and spermine. When the mutants were cultured in the absence of polyamines, a continuously declining growth rate was observed.

MeSH Terms
Adenosylmethionine Decarboxylase/metabolism Genes Genetic Complementation Test Genetic Linkage Ornithine/biosynthesis Ornithine Decarboxylase/metabolism Putrescine/biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism Spermidine/biosynthesis Spermine/biosynthesis
Chemicals
Spermine Ornithine Ornithine Decarboxylase Adenosylmethionine Decarboxylase Spermidine Putrescine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Whitney P A
Morris D R
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.1.214-220.1978
Published
1978-04-00
Pages
214-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222237
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.1.214-220.1978 PMC Full Text View journal details
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