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PMID: 3487284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An animal source for the ROB-1 beta-lactamase of Haemophilus influenzae type b.

Antimicrobial agents and chemotherapy ·Vol. 29 ·No. 2 ·1986-02-00 ·Pages 212-5

Medeiros AA, Levesque R, Jacoby GA

Abstract

The most common cause of ampicillin resistance in Haemophilus influenzae type b is production of TEM-1 beta-lactamase; however, a novel enzyme with a similar substrate profile but a quite different isoelectric point has also been described. This beta-lactamase, designated ROB-1, has not been found previously in any other organism. In a survey of 46 ampicillin-resistant H. influenzae type b isolates, we found a second human isolate that produces ROB-1 and discovered that ampicillin-resistant isolates of the porcine pathogen Haemophilus pleuropneumoniae also produced ROB-1. In both Haemophilus species ROB-1 production was determined by plasmids that had considerable DNA sequence homology. However, the ROB-1 and TEM-1 beta-lactamase genes were not related. Our findings suggest that this form of ampicillin resistance has an animal reservoir and that conditions fostering its prevalence in animal strains may play a role in the spread of resistance to human pathogens.

MeSH Terms
Ampicillin/pharmacology Animals Genes, Bacterial Haemophilus/drug effects,enzymology,genetics Haemophilus Infections/microbiology Haemophilus influenzae/drug effects,enzymology,genetics Humans Isoelectric Point Meningitis, Haemophilus/microbiology Nucleic Acid Hybridization Penicillin Resistance R Factors Swine beta-Lactamases/biosynthesis,genetics
Chemicals
Ampicillin beta-Lactamases beta-lactamase TEM-1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Medeiros A A
Levesque R
Jacoby G A
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16 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.29.2.212
Published
1986-02-00
Pages
212-5
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC176379
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/AAC.29.2.212 PMC Full Text View journal details
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