Idiotype-directed interactions during ontogeny play a major role in the establishment of the adult B cell repertoire.

IgM hybridomas derived from perinatal B cells show a high degree of auto-reactivity and many had demonstrable anti-idiotypic reactivities by binding studies. Selected multispecific antibodies were also shown to have potent idiotype-specific biological activities and if administered at appropriate stages of development could dramatically alter the responses of these mice when challenged with appropriate antigens in adult life. The results obtained suggest that idiotype-directed interactions between neonatal B cells play an important role in the early establishment of the B cell repertoire which is subsequently expressed in adult mice.