Home LiteratureArticle Details
PMID: 3495310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endothelium-derived relaxing factor inhibits in vitro platelet aggregation.

British journal of pharmacology ·Vol. 90 ·No. 4 ·1987-04-00 ·Pages 687-92

Furlong B, Henderson AH, Lewis MJ, Smith JA

Abstract

We studied the effects of endothelium-derived relaxing factor (EDRF), bovine retractor penis muscle inhibitory factor and sodium nitroprusside, three stimulants of guanylate cyclase, on the in vitro aggregation of washed human platelets. Platelet aggregation induced either by collagen or by the thromboxane A2 analogue U46619 was inhibited by all three agents. The anti-aggregatory effect of each agent was inhibited by haemoglobin. The anti-aggregatory effect of EDRF was potentiated by superoxide dismutase. These findings are discussed in relation to a potential role for EDRF in haemostasis.

MeSH Terms
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid Hemoglobins/pharmacology Humans In Vitro Techniques Nitric Oxide Nitroprusside/pharmacology Platelet Aggregation/drug effects Prostaglandin Endoperoxides, Synthetic/pharmacology Vasodilator Agents/pharmacology
Chemicals
Hemoglobins Prostaglandin Endoperoxides, Synthetic Vasodilator Agents Nitroprusside Nitric Oxide 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Furlong B
Henderson A H
Lewis M J
Smith J A
References (26)
26 references, click to expand
  1. Modulation of human platelet adenylate cyclase by prostacyclin (PGX).
    Prostaglandins. 1977 Mar;13(3):377-88 PMID: 191876
  2. Effects of prostacyclin (PGX) on cyclic AMP concentrations in human platelets.
    Prostaglandins. 1977 Mar;13(3):389-97 PMID: 191877
  3. Sodium nitroprusside and other smooth muscle-relaxants increase cyclic GMP levels in rat ductus deferens.
    Nature. 1977 Feb 24;265(5596):750-1 PMID: 193029
  4. Localisation and stimulation of prostacyclin production in vascular cells.
    Nature. 1978 Feb 9;271(5645):549-51 PMID: 340965
  5. Guanylate cyclase: activation by azide, nitro compounds, nitric oxide, and hydroxyl radical and inhibition by hemoglobin and myoglobin.
    Adv Cyclic Nucleotide Res. 1978;9:145-58 PMID: 27076
  6. The inhibitory material in extracts from the bovine retractor penis muscle is not an adenine nucleotide.
    Br J Pharmacol. 1979 Nov;67(3):327-8 PMID: 497534
  7. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine.
    Nature. 1980 Nov 27;288(5789):373-6 PMID: 6253831
  8. A smooth muscle inhibitory material from the bovine retractor penis and rat anococcygeus muscles.
    J Physiol. 1980 Dec;309:55-64 PMID: 6114173
  9. Inhibitory action of guanosine 3', 5'-monophosphate on thrombin-induced phosphatidylinositol turnover and protein phosphorylation in human platelets.
    Biochem Biophys Res Commun. 1981 Jul 16;101(1):61-7 PMID: 6269550
  10. Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries.
    J Physiol. 1981 Jul;316:347-55 PMID: 7320872
  11. Prostacyclin prolongs viability of washed human platelets.
    Biochim Biophys Acta. 1982 Sep 17;718(1):60-5 PMID: 6291622
  12. The interaction of sodium nitroprusside with human endothelial cells and platelets: nitroprusside and prostacyclin synergistically inhibit platelet function.
    Circulation. 1982 Dec;66(6):1299-307 PMID: 6291803
  13. Oxyhaemoglobin blocks non-adrenergic non-cholinergic inhibition in the bovine retractor penis muscle.
    Eur J Pharmacol. 1982 Nov 19;85(2):221-4 PMID: 7151869
  14. Agonist-induced endothelium-dependent relaxation in rat thoracic aorta may be mediated through cGMP.
    Circ Res. 1983 Mar;52(3):352-7 PMID: 6297832
  15. Endothelium-induced relaxation by acetylcholine associated with larger rises in cyclic GMP in coronary arterial strips.
    J Cyclic Nucleotide Res. 1982;8(6):409-19 PMID: 6309931
  16. Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.
    Biochem J. 1983 Jul 15;214(1):273-6 PMID: 6311177
  17. Pharmacological interactions between prostacyclin and thromboxanes.
    Br Med Bull. 1983 Jul;39(3):232-8 PMID: 6313114
  18. Stimulation of endothelial prostacyclin production plays no role in endothelium-dependent relaxation of the pig aorta.
    Br J Pharmacol. 1983 Sep;80(1):179-86 PMID: 6418255
  19. Vasodilatation by acetylcholine is endothelium-dependent: a study by sonomicrometry in canine femoral artery in vivo.
    J Physiol. 1983 Nov;344:209-22 PMID: 6197520
  20. The nature of endothelium-derived vascular relaxant factor.
    Nature. 1984 Apr 12-18;308(5960):645-7 PMID: 6424031
  21. Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle.
    Br J Pharmacol. 1984 Apr;81(4):665-74 PMID: 6326922
  22. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta.
    J Pharmacol Exp Ther. 1985 Mar;232(3):708-16 PMID: 2983068
  23. Autoradiographic localization of beta-adrenoceptor subtypes in guinea-pig kidney.
    Br J Pharmacol. 1985 Jun;85(2):341-8 PMID: 2992660
  24. Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor.
    Nature. 1986 Apr 3-9;320(6061):454-6 PMID: 3007998
  25. Endothelium-dependent inhibition of platelet aggregation.
    Br J Pharmacol. 1986 Jun;88(2):411-5 PMID: 3089351
  26. The regeneration of aortic endothelium.
    J Pathol Bacteriol. 1958 Jan;75(1):133-43 PMID: 13576293
Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1987.tb11221.x
Published
1987-04-00
Pages
687-92
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1917198
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1987.tb11221.x PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2