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PMID: 3496416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Depletion of RT6.1+ T lymphocytes induces diabetes in resistant biobreeding/Worcester (BB/W) rats.

The Journal of experimental medicine ·Vol. 166 ·No. 2 ·1987-08-01 ·Pages 461-75

Greiner DL, Mordes JP, Handler ES, Angelillo M, Nakamura N, Rossini AA

Abstract

To investigate the role of RT6+ T cells in the pathogenesis of diabetes in BB/W rats, we treated animals from the diabetes-resistant (DR) subline with anti-RT6.1 lymphocytotoxic mAb. This depleted greater than 95% of peripheral RT6+ T cells but did not substantially reduce levels of circulating T cells or the in vitro response of spleen cells to mitogen. Treatment of 30-d-old DR BB/W rats in this way: induced insulitis and diabetes, rendered nondiabetic RT6-depleted DR rats susceptible to the adoptive transfer of diabetes by spleen cells from acutely diabetic BB/W rats, and yielded DR spleen cell populations capable of the adoptive transfer of diabetes to diabetes-prone (DP) or DR recipients. Treatment of DR rats beginning at 60 d of age failed to produce these effects. These results suggest that both susceptibility and resistance to diabetes in the BB/W rat are in part regulated by the RT6+ T cell subset and provide evidence for the importance of regulatory T lymphocytes in the pathogenesis of autoimmunity and diabetes in BB/W rats.

MeSH Terms
Age Factors Animals Antibodies, Monoclonal Diabetes Mellitus, Experimental/etiology Disease Susceptibility Immunization, Passive Lymphocyte Depletion Rats Rats, Inbred BB Spleen/cytology T-Lymphocytes/physiology
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Greiner D L
Mordes J P
Handler E S
Angelillo M
Nakamura N
Rossini A A
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43 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.166.2.461
Published
1987-08-01
Pages
461-75
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189590
Subset
IM
Grants
NIDDK NIH HHS · DK-25306 · United States
NIDDK NIH HHS · DK-36024 · United States
External Links
PubMed source DOI: 10.1084/jem.166.2.461 PMC Full Text View journal details
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