Expression of Bcl-2 and Bcl-2-Ig fusion transcripts in normal and neoplastic cells.

The Journal of clinical investigation ·Vol. 80 ·No. 5 ·1987-11-00 ·Pages 1512-5

Graninger WB, Seto M, Boutain B, Goldman P, Korsmeyer SJ

Abstract

We examined the expression of the Bcl-2 gene at chromosome segment 18q21, that is translocated into the Ig heavy chain gene locus in t(14;18) bearing lymphomas. Bcl-2, while B cell associated, is expressed in a variety of hematopoietic lineages including T cells. Bcl-2 mRNA levels are high during pre-B cell development, the time at which the t(14;18) translocation occurs, but are down regulated with maturation. Like certain other oncogenes, Bcl-2 is quiescent in resting B cells but up-regulated with B cell activation. Mature B cell lymphomas with a t(14;18) have log-folds more mRNA than matched counterparts without the translocation. A sensitive S1 protection assay revealed that all transcripts in t(14;18) B cells were Bcl-2-Ig fusion mRNAs and originated from the translocated allele. Thus, there is a marked deregulation of Bcl-2 when it is introduced into the Ig locus in t(14;18) lymphomas.

MeSH Terms

B-Lymphocytes/metabolism Cell Differentiation Chromosomes, Human, Pair 14 Chromosomes, Human, Pair 18 Gene Expression Regulation Humans Lymphocyte Activation Lymphocytes/metabolism Lymphoma/genetics Oncogenes RNA, Messenger/genetics T-Lymphocytes/metabolism Transcription, Genetic Translocation, Genetic Tumor Cells, Cultured

Chemicals

RNA, Messenger

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Graninger W B

Department of Medicine, Microbiology and Immunology, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.

Seto M

Boutain B

Goldman P

Korsmeyer S J

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI113235

Published

1987-11-00

Pages

1512-5

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC442413

Subset

External Links

PubMed source DOI: 10.1172/JCI113235 PMC Full Text View journal details

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Expression of Bcl-2 and Bcl-2-Ig fusion transcripts in normal and neoplastic cells.

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