Home LiteratureArticle Details
PMID: 350318 Published · ppublish English Journal Article

Demonstration of the Fc-receptor of blood cells by soluble peroxidase-anti-peroxidase (PAP) complexes.

Blut ·Vol. 36 ·No. 5 ·1978-05-18 ·Pages 263-73

Huhn D, Andreewa P, Rodt H, Thiel E, Eulitz M

Abstract

The Fc-receptor of normal human leukocytes, of CLL-cells, and of hematopoietic cell lines was demonstrated with soluble peroxidase-anti-peroxidase (PAP) complexes. In about 9% of normal lymphocytes an almost continuous, strong labeling of the cell membrane was established. Some of these lymphocytes were characterized by a peculiar uniform fine structure. The percentage of PAP-labeled monocytes was in the range of 25%, neutrophils nearly 100%, eosinophils 0%, CLL-cells 10%. Labeled portions of the membrane were interiorized from monocytes. The lymphoid cell-line Daudi established from a Burkitt's lymphoma appeared almost negative, the cell line K562 established from a myeloid leukemia in 75% of the cells strongly positive. PAP-labeling was not influenced by preincubation with trypsine or with neuraminidase; it was negative when PAP-F(ab)2 was used. Results of PAP-labeling were not always in agreement with EA-rosettes or with agg-Ig.

MeSH Terms
Binding Sites Humans Immunoenzyme Techniques Immunoglobulin Fc Fragments Leukemia, Lymphoid/immunology Leukocytes/immunology Lymphocytes/immunology,ultrastructure Membranes/immunology Microscopy, Electron
Chemicals
Immunoglobulin Fc Fragments
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huhn D
Andreewa P
Rodt H
Thiel E
Eulitz M
References (31)
31 references, click to expand
  1. Quantitation of T-antigenic sites and Ig-determinants on leukemic cells by microphotometric immunoautoradiography. Proof of the clonal origin of thymus-derived lymphocytic leukemias.
    Haematol Blood Transfus. 1977;20:131-45 PMID: 415942
  2. Immune complex receptors on cell surfaces. II. Cytochemical evaluation of their abundance on different immune cells: distribution, uptake, and regeneration.
    Am J Pathol. 1976 Sep;84(3):437-56 PMID: 60883
  3. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer.
    J Cell Biol. 1967 Oct;35(1):213-36 PMID: 6061717
  4. Studies of the human lymphocyte receptor for heat-aggregated or antigen-complexed immunoglobulin.
    J Exp Med. 1974 Aug 1;140(2):508-22 PMID: 4603014
  5. Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants.
    J Exp Med. 1975 Mar 1;141(3):547-60 PMID: 46915
  6. Cell marker analysis in acute monocytic leukemias.
    Blood. 1977 Jun;49(6):895-901 PMID: 324537
  7. The interaction of particulate horseradish peroxidase (HRP)-anti HRP immune complexes with mouse peritoneal macrophages in vitro.
    J Cell Biol. 1972 Dec;55(3):616-34 PMID: 4656704
  8. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance.
    J Exp Med. 1972 Mar 1;135(3):610-26 PMID: 5058234
  9. [Ultrahistochemical studies on the glycocalyx of the thoracic duct lymphocytes].
    Z Zellforsch Mikrosk Anat. 1972;134(1):143-52 PMID: 4634678
  10. Receptors for human gamma G globulin on human neutrophils.
    J Clin Invest. 1970 Dec;49(12):2165-71 PMID: 4991439
  11. The function of macrophages in the immune response.
    Semin Hematol. 1970 Apr;7(2):225-48 PMID: 4910220
  12. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.
    Cancer Res. 1968 Jul;28(7):1300-10 PMID: 4174339
  13. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes.
    J Exp Med. 1973 Dec 1;138(6):1365-78 PMID: 4357682
  14. Electron microscopic studies of human monocyte and lymphocyte interaction with immunoglobulin- and complement-coated erythrocytes.
    Exp Cell Res. 1972 Jan;70(1):161-72 PMID: 4621474
  15. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
    Blood. 1975 Mar;45(3):321-34 PMID: 163658
  16. IgG subclass specificity of human monocyte receptor sites.
    Nature. 1971 Feb 5;229(5284):419-20 PMID: 4926996
  17. Identification of three different human lymphocyte populations by surface markers.
    Transplant Rev. 1973;16:114-62 PMID: 4590767
  18. Lymphocyte binding of aggregated immunoglobulin.
    Scand J Immunol. 1976 Jun;Suppl 5:91-7 PMID: 1088711
  19. An appraisal of Fc receptors on human peripheral blood B and L lymphocytes.
    J Immunol. 1976 Sep;117(3):939-43 PMID: 1085313
  20. The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro.
    J Cell Biol. 1972 Oct;55(1):186-204 PMID: 4347251
  21. Lymphocyte membrane receptors in human lymphoid leukemias.
    Eur J Immunol. 1975 Feb;5(2):89-93 PMID: 135682
  22. [A new type of organelle in peripheral lymphocytes?].
    Dtsch Med Wochenschr. 1968 Nov 1;93(44):2099-100 PMID: 5686333
  23. Disappearance and recovery of human monocyte IgG receptor activity after phagocytosis.
    J Immunol. 1972 Oct;109(4):914-7 PMID: 4672579
  24. Binding of aggregated IgG BY HUMAN B lymphocytes independent of Fc receptors.
    Scand J Immunol. 1974;3(3):375-80 PMID: 4546736
  25. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
    J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
  26. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds.
    Arch Biochem Biophys. 1960 Aug;89:230-44 PMID: 14427334
  27. The interaction between human monocytes and red cells. Specificity for IgG subclasses and IgG fragments.
    J Exp Med. 1970 Dec 1;132(6):1207-15 PMID: 5511570
  28. Surface receptors on human haematopoietic cell lines.
    Clin Exp Immunol. 1976 Sep;25(3):367-76 PMID: 963908
  29. Receptor sites of human monocytes for IgG.
    Int Arch Allergy Appl Immunol. 1968;34(1):18-31 PMID: 4174441
  30. Interaction of aggregated -globulin with B lymphocytes.
    J Exp Med. 1972 Jul 1;136(1):191-6 PMID: 4113510
  31. Immune complex receptors on cell surface. I. Ultrastructural demonstration of macrophages.
    J Histochem Cytochem. 1976 Aug;24(8):948-55 PMID: 60441
Article Info
Journal
Blut
Abbr.
Blut
ISSN
0006-5242
DOI
10.1007/BF01880677
Published
1978-05-18
Pages
263-73
Language
English
Region
Germany
NLM ID
0173401
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01880677
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2