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PMID: 350837 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase.

Journal of bacteriology ·Vol. 134 ·No. 3 ·1978-06-00 ·Pages 1039-45

Duncan BK, Rockstroh PA, Warner HR

Abstract

A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA.

MeSH Terms
Chromosome Mapping Coliphages/analysis,growth & development DNA Repair DNA, Viral/analysis Deoxyuracil Nucleotides Escherichia coli/genetics Genes Mutation N-Glycosyl Hydrolases/genetics Uracil/analysis
Chemicals
DNA, Viral Deoxyuracil Nucleotides Uracil N-Glycosyl Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Duncan B K
Rockstroh P A
Warner H R
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.3.1039-1045.1978
Published
1978-06-00
Pages
1039-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222353
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.3.1039-1045.1978 PMC Full Text View journal details
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