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PMID: 350849 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Variations among glyV-derived glycine tRNA suppressors of glutamic acid codons.

Journal of bacteriology ·Vol. 134 ·No. 3 ·1978-06-00 ·Pages 801-7

Murgola EJ, Prather NE, Hadley KH

Abstract

Glutamic acid codon suppressors in 18 isogenic strains of Escherichia coli have been further characterized as to map location, dominance, growth rates in various media, suppression of the GAG codon, and tRNA profiles after reversed-phase column chromatography. In general the evidence supports the conclusion that all of these suppressors are due to mutations in glyV55, the gene for a GGA/G-reading mutant form of glyV tRNA, and that they represent several different classes that may correspond to at least as many different nucleotide changes. Furthermore, 17 of the 18 suppressors can coexist in a haploid genome with a glyT suppressor that is devoid of GGA-reading ability. This result indicates the retention by those glyV suppressors of some ability to respond to GGA as well as the acquisition of the ability to read GAA, and suggests the possibility of "wobble" in the middle position of the anticodons of those tRNA's.

MeSH Terms
Chromosome Mapping Codon Escherichia coli/genetics Glutamates/genetics Glycine/genetics Mutation RNA, Bacterial/genetics RNA, Messenger RNA, Transfer/genetics Suppression, Genetic
Chemicals
Codon Glutamates RNA, Bacterial RNA, Messenger RNA, Transfer Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murgola E J
Prather N E
Hadley K H
References (22)
22 references, click to expand
  1. A BIOCHEMICAL AND GENETIC STUDY OF REVERSION WITH THE A-GENE A-PROTEIN SYSTEM OF ESCHERICHIA COLI TRYPTOPHAN SYNTHETASE.
    Genetics. 1963 Aug;48:1065-83 PMID: 14050802
  2. Codon--anticodon pairing: the wobble hypothesis.
    J Mol Biol. 1966 Aug;19(2):548-55 PMID: 5969078
  3. Three different missense suppressor mutations affecting the tRNA GGG Gly species of Escherichia coli.
    J Bacteriol. 1974 Feb;117(2):351-9 PMID: 4590463
  4. Glycyl transfer ribonucleic acid synthetase from Escherichia coli: purification, properties, and substrate binding.
    Biochemistry. 1974 Mar 26;13(7):1338-48 PMID: 4594761
  5. Genetically and chemically derived missense suppressor transfer RNA's with altered enzymic aminoacylation rates.
    J Mol Biol. 1968 Dec 14;38(2):201-16 PMID: 4330697
  6. Normal and mutant glycine transfer RNAs.
    Nat New Biol. 1971 Oct 27;233(43):274-7 PMID: 4941781
  7. Frameshift suppression: a nucleotide addition in the anticodon of a glycine transfer RNA.
    Nat New Biol. 1973 Apr 25;242(121):230-4 PMID: 4573868
  8. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  9. Selection for new amino acids at position 211 of the tryptophan synthetase alpha chain of Escherichia coli.
    J Mol Biol. 1974 Jul 15;86(4):775-84 PMID: 4610146
  10. Molecular mechanism for missense suppression in E. coli.
    Nature. 1974 Aug 2;250(465):412-4 PMID: 4604787
  11. Structural interactions between amino acid residues at positions 22 and 211 in the tryptophan synthetase alpha chain of Escherichia coli.
    J Bacteriol. 1974 Feb;117(2):444-8 PMID: 4590468
  12. Suppression of glutamic acid codons by mutant glycine transfer ribonucleic acid.
    J Bacteriol. 1974 Feb;117(2):439-43 PMID: 4590467
  13. Nucleotide sequence studies of normal and genetically altered glycine transfer ribonucleic acids from Escherichia coli.
    J Biol Chem. 1975 Jul 25;250(14):5530-41 PMID: 167016
  14. Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation.
    J Mol Biol. 1970 Sep 28;52(3):571-84 PMID: 4923752
  15. Influence of chromosome structure on the frequency of tonB trp deletions in Escherichia coli.
    J Bacteriol. 1971 Mar;105(3):864-72 PMID: 4930422
  16. Tryptophan transfer RNA as the UGA suppressor.
    J Mol Biol. 1971 Jun 14;58(2):439-58 PMID: 4933412
  17. Amino acid replacements and the genetic code.
    Cold Spring Harb Symp Quant Biol. 1966;31:151-62 PMID: 4866372
  18. Multiple gene loci for a single species of glycine transfer ribonucleic acid.
    J Bacteriol. 1975 May;122(2):492-501 PMID: 1092653
  19. Allosteric mechanism for codon-dependent tRNA selection on ribosomes.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4248-51 PMID: 1060103
  20. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.
    Biochim Biophys Acta. 1971 Feb 11;228(3):770-4 PMID: 5572614
  21. T4 bacteriophage mutants suppressible by a missense suppressor which inserts glycine in place of arginine for the codon AGA.
    J Virol. 1968 Sep;2(9):905-14 PMID: 5725322
  22. MUTATIONALLY INDUCED AMINO ACID SUBSTITUTIONS IN A TRYPTIC PEPTIDE OF THE TRYPTOPHAN SYNTHETASE A PROTEIN.
    J Biol Chem. 1965 Feb;240:679-89 PMID: 14275122
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.3.801-807.1978
Published
1978-06-00
Pages
801-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222326
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.3.801-807.1978 PMC Full Text View journal details
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