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PMID: 3510435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Unusual association of beta 2-microglobulin with certain class I heavy chains of the murine major histocompatibility complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 2 ·1986-01-00 ·Pages 432-6

Bushkin Y, Tung JS, Pinter A, Michaelson J, Boyse EA

Abstract

Class I products of the major histocompatibility complex (MHC) comprise a heavy chain of about 45 kDa noncovalently linked to a 12-kDa beta 2-microglobulin (beta 2m) light chain encoded on a different chromosome. We find that class I products of some mouse strains include an additional 62-kDa molecule which on the following evidence consists of a heavy chain linked covalently with beta 2m. Production of the 62-kDa protein invariably accorded with the occurrence of cysteine at position 121 of the heavy chain (Kb,Kbm1,Kbm3,Dd, and Ld). Substitution of arginine at position 121 invariably accorded with absence of the 62-kDa protein (Kbm6,Kbm7,Kbm9,Kd, and Db). On the basis of observed production versus nonproduction of the 62-kDa molecule, predictions are made regarding residue 121 in class I products for which this is not yet known; namely, Kk, Ks, and Dk, which produce the 62-kDa molecule, as compared with Kj, Qa-2, and TL, which do not. Reported differences in immunologic reactivity between Kb mutant strains with Arg-121 in place of Cys-121 imply that the occurrence of 62-kDa class I products in mice of Cys-121 genotype has functional consequences.

MeSH Terms
Alleles Animals Disulfides H-2 Antigens/genetics Macromolecular Substances Major Histocompatibility Complex Membrane Proteins/metabolism Mice Molecular Weight Structure-Activity Relationship beta 2-Microglobulin/genetics,metabolism
Chemicals
Disulfides H-2 Antigens Macromolecular Substances Membrane Proteins beta 2-Microglobulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bushkin Y
Tung J S
Pinter A
Michaelson J
Boyse E A
References (30)
30 references, click to expand
  1. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  2. A nonpolymorphic class I gene in the murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):139-44 PMID: 6420070
  3. Biochemical characterization of the human T6 antigen: a comparison between T6 and murine TL.
    Mol Immunol. 1984 Oct;21(10):821-9 PMID: 6438495
  4. The complete amino acid sequence of beta 2-microglobulin.
    Biochemistry. 1973 Nov 20;12(24):4811-22 PMID: 4586824
  5. Notable diversity in peptide composition of murine H-2K and H-2D alloantigens.
    Biochemistry. 1974 Jul 16;13(15):3174-8 PMID: 4841060
  6. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  7. A mouse plasma substance carrying beta2-microglobulin activity and lacking in H-2 alloantigenic activity.
    J Immunogenet. 1976 Apr;3(2):123-34 PMID: 58948
  8. Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family.
    Nature. 1977 May 5;267(5606):23-8 PMID: 193039
  9. Interaction between the third complement protein and cell surface macromolecules.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2701-5 PMID: 268620
  10. Primary structure of murine major histocompatibility complex alloantigens: isolation, biochemical characterization, and preliminary alignment of CNBr fragments from the H-2Ib glycoprotein.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2909-13 PMID: 275861
  11. Isolation and partial characterization of a beta2-microglobulin-containing, H-2 antigen-like murine serum protein.
    Biochemistry. 1978 Oct 31;17(22):4794-801 PMID: 83153
  12. Fragmentation of the human transplantation antigen heavy chain by limited proteolysis, acid cleavage, and cyanogen bromide treatment.
    Biochemistry. 1979 Apr 3;18(7):1322-8 PMID: 371676
  13. Characterization of alkylamine-sensitive site in alpha 2-macroglobulin.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4313-6 PMID: 92026
  14. Synthesis and processing of molecules bearing thymus leukemia antigen.
    J Exp Med. 1979 Oct 1;150(4):777-91 PMID: 315985
  15. Genetic polymorphism of murine beta 2-microglobulin detected biochemically.
    Immunogenetics. 1980 Jul;11(1):93-5 PMID: 6160099
  16. Mouse histocompatibility genes: structure and organisation of a Kd gene.
    EMBO J. 1983;2(2):245-54 PMID: 11894934
  17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  18. Biochemistry of the gene products from murine MHC mutants.
    Annu Rev Genet. 1980;14:241-77 PMID: 7011174
  19. Allelic forms of beta 2-microglobulin in the mouse.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7395-9 PMID: 6164059
  20. Primary structure of a murine transplantation antigen.
    Nature. 1981 May 7;291(5810):35-9 PMID: 7231522
  21. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  22. Structure of murine major histocompatibility complex alloantigens: identification of cysteine residues involved in two intrachain disulfide bonds of H-2K.
    Mol Immunol. 1981 Oct;18(10):883-8 PMID: 7335082
  23. Amino acid sequence around the thiol and reactive acyl groups of human complement component C4.
    Biochem J. 1981 Nov 1;199(2):359-70 PMID: 6978711
  24. Primary structure of the H-2Db alloantigen. II. Additional amino acid sequence information, localization of a third site of glycosylation and evidence for K and D region specific sequences.
    Immunogenetics. 1982;16(1):11-22 PMID: 7118212
  25. Intramolecular organization of Class I H-2 MHC antigens; localization of the alloantigenic determinants and the beta 2 m binding site to different regions of the H-2 Kb glycoprotein.
    J Immunol. 1983 Mar;130(3):1419-25 PMID: 6185585
  26. Biochemical studies of H-2K antigens from a group of related mutants. I. Identification of a shared mutation in B6-H-2bm5 and B6-H-2bm16.
    Immunogenetics. 1983;17(1):19-29 PMID: 6826207
  27. Biochemical studies of H-2K antigens from a group of related mutants. II. Identification of a shared mutation in B6-H-2bm6, B6.C-H-2bm7, and B6.C-H-2bm9.
    Immunogenetics. 1983;17(1):31-41 PMID: 6826208
  28. H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. VI. H-2Kb mutants differentially regulate the immune response to the H-4.2 alloantigen.
    Immunogenetics. 1983;17(2):109-23 PMID: 6402447
  29. Genetics of beta-2 microglobulin in the mouse.
    Immunogenetics. 1983;17(3):219-60 PMID: 6187676
  30. Evidence for presence of an internal thiolester bond in third component of human complement.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5764-8 PMID: 6934510
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.2.432
Published
1986-01-00
Pages
432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322873
Subset
IM
Grants
PHS HHS · A121579 · United States
NCI NIH HHS · CA20473 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.2.432 PMC Full Text View journal details
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