Home LiteratureArticle Details
PMID: 3514461 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli.

Infection and immunity ·Vol. 52 ·No. 1 ·1986-04-00 ·Pages 334-6

Baldini MM, Nataro JP, Kaper JB

Abstract

pMAR2, a 60-megadalton plasmid encoding localized HEp-2 adherence in enteropathogenic Escherichia coli, was mapped with BamHI, HindIII, and SalI. Deletion and insertion mutants were constructed and used to define a potential DNA probe. Preliminary results indicate that this probe is sensitive and specific for the genes encoding the enteropathogenic E. coli adherence factor.

MeSH Terms
Adhesiveness Chromosome Mapping Escherichia coli/genetics,pathogenicity Genes, Bacterial Humans Nucleic Acid Hybridization Plasmids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baldini M M
Nataro J P
Kaper J B
References (17)
17 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  3. Role of Escherichia coli colonisation factor antigen in acute diarrhoea.
    Lancet. 1978 Jul 22;2(8082):181-4 PMID: 78384
  4. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  6. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
    J Infect Dis. 1980 Dec;142(6):892-8 PMID: 7007526
  7. Mapping of a plasmid, coding for colonization, factor antigen I and heat-stable enterotoxin production, isolated from an enterotoxigenic strain of Escherichia coli.
    J Bacteriol. 1982 Jan;149(1):264-75 PMID: 6274845
  8. A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants.
    Gastroenterology. 1982 Aug;83(2):441-54 PMID: 7044882
  9. Enteropathogenic Escherichia coli serotypes and endemic diarrhea in infants.
    Infect Immun. 1983 Feb;39(2):586-9 PMID: 6339384
  10. Plasmid-mediated adhesion in enteropathogenic Escherichia coli.
    J Pediatr Gastroenterol Nutr. 1983;2(3):534-8 PMID: 6352891
  11. Enteropathogenic Escherichia coli of classic serotypes associated with infant diarrhea: epidemiology and pathogenesis.
    Epidemiol Rev. 1984;6:31-51 PMID: 6386503
  12. Plasmid-mediated factors conferring diffuse and localized adherence of enteropathogenic Escherichia coli.
    Infect Immun. 1985 May;48(2):378-83 PMID: 2859247
  13. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor.
    J Infect Dis. 1985 Sep;152(3):550-9 PMID: 2863318
  14. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe.
    J Infect Dis. 1985 Sep;152(3):560-5 PMID: 2863319
  15. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  16. Pathogenesis of Escherichia coli diarrhea.
    N Engl J Med. 1971 Jul 1;285(1):1-9 PMID: 4996788
  17. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.
    Infect Immun. 1975 Sep;12(3):656-67 PMID: 1100526
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
1986-04-00
Pages
334-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262241
Subset
IM
Grants
PHS HHS · N01-ALL2666 · United States
NIAID NIH HHS · R22-AI19716 · United States
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]