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PMID: 351614 Published · ppublish English Journal Article

Transformation-dependent secretion of a low molecular weight protein by murine fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 6 ·1978-06-00 ·Pages 2767-71

Gottesman MM

Abstract

A protocol has been devised to radiolabel proteins secreted by murine fibroblasts in vitro. A radiolabeled polypeptide of molecular weight 35,000 is released into medium in relatively large amounts by transformed cells and in much smaller amounts by nontransformed fibroblasts. This major excreted polypeptide (MEP) is found in the medium of spontaneously transformed mouse cells and in the medium of mouse cells transformed by a DNA tumor virus, RNA tumor viruses, or methylcholanthrene. The appearance of MEP appears to be well correlated with anchorage independence in these transformed cells. MEP can be localized within the cytoplasm of transformed but not untransformed cells by indirect immunofluorescence. The presence of MEP within murine fibroblasts or in their culture medium serves as a novel biochemical marker of transformation. A biological role for this protein has not been assigned.

MeSH Terms
Cell Line Cell Transformation, Neoplastic/metabolism Cell Transformation, Viral Cytoplasm/metabolism Fluorescent Antibody Technique Methylcholanthrene Molecular Weight Neoplasm Proteins/metabolism
Chemicals
Neoplasm Proteins Methylcholanthrene
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gottesman M M
References (20)
20 references, click to expand
  1. Mechanism of the decrease in the major cell surface protein of chick embryo fibroblasts after transformation.
    Cell. 1977 Aug;11(4):957-69 PMID: 196771
  2. Anchorage and growth regulation in normal and virus-transformed cells.
    Int J Cancer. 1968 Sep 15;3(5):683-93 PMID: 5749478
  3. Synthesis of types I and III procollagen and collagen by monkey aortic smooth muscle cells in vitro.
    Biochemistry. 1977 Jul 12;16(14):3243-9 PMID: 407926
  4. Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1083-6 PMID: 772678
  5. The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts.
    Arch Biochem Biophys. 1972 Sep;152(1):318-28 PMID: 4342111
  6. Morphologic revertants of murine sarcoma virus transformed nonproducer BALB-3T3: selective techniques for isolation and biologic properties in vitro and in vivo.
    Virology. 1974 Feb;57(2):339-46 PMID: 4361451
  7. Alteration of cell-surface proteins by viral transformation and by proteolysis.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4 PMID: 4361679
  8. MIF-like activity in simian virus 40-transformed 3T3 fibroblast cultures.
    Science. 1974 Sep 13;185(4155):955-7 PMID: 4377757
  9. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses.
    J Exp Med. 1973 Jan 1;137(1):112-26 PMID: 4347288
  10. A factor from a transformed cell line that affects cell migration.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):369-72 PMID: 4510281
  11. Isolation of a major cell surface glycoprotein from fibroblasts.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3492-6 PMID: 4530317
  12. Plasminogen-independent fibrinolysis by proteases produced by transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1132-6 PMID: 165484
  13. Loss of ability to synthesize collagen in fibroblasts transformed by rous sarcoma virus.
    J Natl Cancer Inst. 1975 Oct;55(4):807-10 PMID: 171426
  14. Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4435-9 PMID: 172908
  15. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  16. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  17. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts.
    J Cell Physiol. 1973 Apr;81(2):153-60 PMID: 4735141
  18. Tumor angiogenesis activity in cells grown in tissue culture.
    Cancer Res. 1976 Jan;36(1):110-4 PMID: 1247990
  19. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  20. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399-403 PMID: 198783
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.6.2767
Published
1978-06-00
Pages
2767-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392645
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.6.2767 PMC Full Text View journal details
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