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PMID: 3517869 Published · ppublish English Journal Article

Structure and sequence of the human c-sis/platelet-derived growth factor 2 (SIS/PDGF2) transcriptional unit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2392-6

Rao CD, Igarashi H, Chiu IM, Robbins KC, Aaronson SA

Abstract

The structure of the normal human c-sis/platelet-derived growth factor 2 (SIS/PDGF2) transcript was determined by a combination of cDNA cloning, nuclease S1 mapping, and primer extension. Nucleotide sequence analysis revealed that the 3373-nucleotide SIS/PDGF2 mRNA contained only a 723-base-pair (bp) coding sequence for the PDGF2 precursor polypeptide. The coding sequence was flanked by long 5' (1022 bp) and 3' (1625 bp) untranslated regions. The 5' noncoding region, as well as upstream flanking genomic sequences, contained clusters of specific short repeat sequences. A consensus transcriptional promoter sequence, TATAAA, was identified 24 bp upstream of the mRNA start site and an enhancer-like "TG element" was detected about 180 bp downstream from the site of polyadenylylation. These findings identify putative regulatory elements of the SIS/PDGF2 gene.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Enhancer Elements, Genetic Genes Humans Placenta Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogenes RNA, Messenger/genetics Transcription, Genetic
Chemicals
Proto-Oncogene Proteins RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rao C D
Igarashi H
Chiu I M
Robbins K C
Aaronson S A
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2392
Published
1986-04-00
Pages
2392-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323303
Subset
IM
Databases
GENBANK
M12783
External Links
PubMed source DOI: 10.1073/pnas.83.8.2392 PMC Full Text View journal details
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