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PMID: 3517877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein fatty acid acylation: enzymatic synthesis of an N-myristoylglycyl peptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 9 ·1986-05-00 ·Pages 2812-6

Towler D, Glaser L

Abstract

Incubation of Saccharomyces cerevisiae strain JR153 with either [3H]myristate or [3H]palmitate demonstrates the synthesis of proteins that contain covalently bound fatty acids. A unique set of proteins is labeled by each fatty acid. Detailed analysis of a 20-kDa protein labeled with myristic acid demonstrates that myristate is linked to the amino-terminal glycine. We describe an enzymatic activity in yeast that will transfer myristic acid to the amino terminus of the octapeptide Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg, whose sequence was derived from a known N-myristoylated acyl protein, the catalytic subunit of cAMP-dependent protein kinase of bovine cardiac muscle. The acylation reaction is dependent on ATP and CoA, is enriched in a crude membrane fraction, and will use myristate but not palmitate as the acyl donor. Specificity of the glycyl peptide substrate is demonstrated by the observation that other glycyl peptides do not competitively inhibit myristoylation of Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg.

MeSH Terms
Acylation Adenosine Triphosphate/pharmacology Amino Acid Sequence Coenzyme A Ligases/pharmacology Glycine/metabolism Myristic Acid Myristic Acids/metabolism Palmitic Acid Palmitic Acids/metabolism Peptides/metabolism Saccharomyces cerevisiae/metabolism Tritium
Chemicals
Myristic Acids Palmitic Acids Peptides Myristic Acid Tritium Palmitic Acid Adenosine Triphosphate Coenzyme A Ligases Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Towler D
Glaser L
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.9.2812
Published
1986-05-00
Pages
2812-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323396
Subset
IM
Grants
NIGMS NIH HHS · GM 18405 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.9.2812 PMC Full Text View journal details
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