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PMID: 3518425 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Computer prediction of peptide maps: assignment of polypeptides to human and mouse mitochondrial DNA genes by analysis of two-dimensional-proteolytic digest gels.

American journal of human genetics ·Vol. 38 ·No. 4 ·1986-04-00 ·Pages 461-81

Wallace DC, Yang JH, Ye JH, Lott MT, Oliver NA, McCarthy J

Abstract

We have prepared a computer program that predicts complete and partial peptide maps from amino acid sequences. The program fragments amino acid sequences at designated cleavage sites and calculates the molecular weight and relative labeling of each peptide. These data are graphed as log molecular weight of the original protein (X-axis) vs. log molecular weight of the component peptides (Y-axis). The program is interactive, permitting adjustment of a number of graphic parameters and alteration of the position of proteins in the first dimension to accommodate aberrations in protein mobility. The program has been used to predict the V8 protease peptide maps of the 13 open reading frames (ORFs) identified in the human and the mouse mitochondrial DNA (mtDNA) sequences. The results were compared to the V8 protease peptide maps obtained for mouse and human mitochondrially synthesized proteins by two-dimensional proteolytic digest gels. A high correlation was observed between the predicted and observed peptide maps. These results suggest the assignment of several proteins to mtDNA genes.

MeSH Terms
Amino Acid Sequence Animals Computers DNA, Mitochondrial/genetics Electrophoresis, Polyacrylamide Gel Humans Hydrolysis Mice Peptide Hydrolases Peptides Software Species Specificity
Chemicals
DNA, Mitochondrial Peptides Peptide Hydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wallace D C
Yang J H
Ye J H
Lott M T
Oliver N A
McCarthy J
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30 references, click to expand
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1986-04-00
Pages
461-81
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684801
Subset
IM
Grants
NIGMS NIH HHS · GM33022 · United States
NINDS NIH HHS · NS21328 · United States
External Links
PubMed source PMC Full Text View journal details
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