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PMID: 3518706 Published · ppublish English Journal Article

The serC-aro A operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways.

The Biochemical journal ·Vol. 234 ·No. 1 ·1986-02-15 ·Pages 49-57

Duncan K, Coggins JR

Abstract

Sub-cloning experiments aimed at precisely locating the E. coli aroA gene, which encodes the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate synthase, showed that in certain constructions, which remain capable of complementing an auxotrophic aroA mutation, expression of aroA is reduced. DNA sequence analysis revealed that a sequence approx. 1200 base pairs (bp) upstream of aroA is necessary for its expression. An open reading frame was identified in this region which encodes a protein of 362 amino acids with a calculated Mr of 39,834 and which ends 70 bp before the start of the aroA coding sequence. This gene has been identified as serC, the structural gene for 3-phosphoserine aminotransferase, an enzyme of the serine biosynthetic pathway. Both genes are expressed as a polycistronic message which is transcribed from a promotor located 58 bp upstream of serC. Evidence is presented which confirms that the aroA and serC genes constitute an operon which has the novel feature of encoding enzymes from two different amino acid biosynthetic pathways.

MeSH Terms
3-Phosphoshikimate 1-Carboxyvinyltransferase Alkyl and Aryl Transferases Amino Acids/biosynthesis Base Sequence Cloning, Molecular DNA/genetics Escherichia coli/enzymology,genetics Genes Operon Promoter Regions, Genetic Transaminases/genetics Transcription, Genetic Transferases/genetics
Chemicals
Amino Acids DNA Transferases Alkyl and Aryl Transferases 3-Phosphoshikimate 1-Carboxyvinyltransferase Transaminases phosphoserine aminotransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Duncan K
Coggins J R
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47 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2340049
Published
1986-02-15
Pages
49-57
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146525
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2340049 PMC Full Text View journal details
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