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PMID: 3521585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural and kinetic studies on beta-lactamase K1 from Klebsiella aerogenes.

The Biochemical journal ·Vol. 234 ·No. 2 ·1986-03-01 ·Pages 343-7

Emanuel EL, Gagnon J, Waley SG

Abstract

beta-Lactamase K1 from Klebsiella aerogenes 1082E hydrolyses both penicillins and cephalosporins comparably and is inhibited by mercurials but not by cloxacillin. These properties distinguish it from those other beta-lactamases that have been allotted to classes on the basis of their amino sequences. beta-Lactamase K1 has been isolated by affinity chromatography; its composition shows resemblances to class A beta-lactamases. Moreover, the N-terminal sequence is similar to those of class A beta-lactamases: there is about 30% identity over the first 32 residues. Furthermore, a putative active-site octapeptide has been isolated and its sequence is similar to the region around the active-site serine residue in class A beta-lactamases. There is one thiol group in beta-lactamase K1; it is not essential for activity. The pH-dependence of kcat. and kcat./Km for the hydrolysis of benzylpenicillin by beta-lactamase K1 were closely similar, suggesting that the rate-determining step is cleavage of the beta-lactam ring.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Binding Sites Hydrogen-Ion Concentration Isoenzymes/metabolism Kinetics Klebsiella pneumoniae/enzymology Penicillinase/metabolism Protein Denaturation Substrate Specificity
Chemicals
Amino Acids Isoenzymes Penicillinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Emanuel E L
Gagnon J
Waley S G
References (30)
30 references, click to expand
  1. The purification and some properties of a -lactamase sensitive to inhibition by p-chloromercuribenzoate.
    Biochim Biophys Acta. 1971 Nov 13;250(2):428-36 PMID: 5143347
  2. Penicillinase from Klebsiella aerogenes. A comparison with penicillinases from gram-positive species.
    Biochem J. 1963 Apr;87:209-14 PMID: 13952353
  3. The beta-lactamases of gram-negative bacteria and their possible physiological role.
    Adv Microb Physiol. 1973;9:31-88 PMID: 4581138
  4. Purification of beta-lactamases on QAE-sephadex.
    Biochim Biophys Acta. 1973 Jun 6;309(2):430-9 PMID: 4731970
  5. A spectrophotometric assay of beta-lactamase action on penicillins.
    Biochem J. 1974 Jun;139(3):789-90 PMID: 4368359
  6. Metal cofactor requirements of beta-lactamase II.
    Biochem J. 1974 Oct;143(1):129-35 PMID: 4219279
  7. The pH-dependence and group modification of beta-lactamase I.
    Biochem J. 1975 Sep;149(3):547-51 PMID: 996
  8. Estimation of the dissociation constants of enzyme-substrate complexes from steady-state measurements. Interpretation of pH-independence of Km.
    Biochem J. 1976 Feb 1;153(2):455-61 PMID: 6011
  9. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  10. Peptide analysis by isoelectric focusing in polyacrylamide gels.
    Anal Biochem. 1978 Mar;85(1):1-14 PMID: 629374
  11. The beta-lactamase stability of a novel beta-lactam antibiotic containing a 7 alpha-methoxyoxacephem nucleus.
    J Antimicrob Chemother. 1980 Jul;6(4):445-53 PMID: 6448832
  12. pH dependence and solvent deuterium oxide kinetic isotope effects on Bacillus cereus beta-lactamase I catalyzed reactions.
    Biochemistry. 1984 Mar 13;23(6):1282-7 PMID: 11494985
  13. A simple test for inactivation of an enzyme during assay.
    Biochim Biophys Acta. 1965 Jul 29;105(1):193-5 PMID: 4221326
  14. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  15. Chemical studies on methionyl-tRNA synthetase from Escherichia coli.
    J Mol Biol. 1970 Sep 14;52(2):165-78 PMID: 4922213
  16. The structure of beta-lactamases.
    Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31 PMID: 6109327
  17. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901 PMID: 6795623
  18. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
    Biochem J. 1981 Apr 1;195(1):15-30 PMID: 6118137
  19. The classification and terminology of enzymes that hydrolyze beta-lactam antibiotics.
    J Infect Dis. 1982 May;145(5):762-5 PMID: 6978915
  20. Half-time analysis of the integrated Michaelis equation. Simulation and use of the half-time plot and its direct linear variant in the analysis of some alpha-chymotrypsin, papain- and fumarase-catalysed reactions.
    Biochem J. 1982 May 1;203(2):351-60 PMID: 7115291
  21. Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
    Antimicrob Agents Chemother. 1982 Sep;22(3):414-20 PMID: 6982680
  22. The acyl-enzyme mechanism of beta-lactamase action. The evidence for class C Beta-lactamases.
    Biochem J. 1982 Nov 1;207(2):315-22 PMID: 6818947
  23. Molecular cloning and nucleotide sequence of the type I beta-lactamase gene from Bacillus cereus.
    Nucleic Acids Res. 1983 Jul 25;11(14):4997-5004 PMID: 6308567
  24. Characterization of the membrane beta-lactamase in Bacillus cereus 569/H/9.
    Biochemistry. 1983 Sep 27;22(20):4647-51 PMID: 6414514
  25. Purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose.
    Biochem J. 1984 Jul 15;221(2):505-12 PMID: 6332621
  26. The active site of the P99 beta-lactamase from Enterobacter cloacae.
    Biochem J. 1984 Oct 1;223(1):271-4 PMID: 6333871
  27. Improved purification and characterization of the OXA-2 beta-lactamase.
    Biochem J. 1984 Dec 15;224(3):1009-13 PMID: 6335398
  28. The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.
    Biochem J. 1985 May 15;228(1):241-8 PMID: 2988516
  29. The production and molecular properties of the zinc beta-lactamase of Pseudomonas maltophilia IID 1275.
    Biochem J. 1985 Aug 1;229(3):791-7 PMID: 3931629
  30. Comparison of the substrate specificities of the -lactamases from Klebsiella aerogenes 1082E and Enterobacter cloacae P99.
    Appl Microbiol. 1972 Apr;23(4):765-9 PMID: 4553144
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2340343
Published
1986-03-01
Pages
343-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146571
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2340343 PMC Full Text View journal details
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