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PMID: 3526285 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Translational regulation of expression of the bacteriophage T4 lysozyme gene.

Nucleic acids research ·Vol. 14 ·No. 14 ·1986-07-25 ·Pages 5813-26

McPheeters DS, Christensen A, Young ET, Stormo G, Gold L

Abstract

The bacteriophage T4 lysozyme gene is transcribed at early and late times after infection of E. coli, but the early mRNA is not translated. DNA sequence analysis and mapping of the 5' ends of the lysozyme transcripts produced at different times after T4 infection show that the early mRNA is initiated some distance upstream from the gene. The early mRNA is not translated because of a stable secondary structure which blocks the translational initiation site. The stable RNA structure has been demonstrated by nuclease protection in vivo. After DNA replication begins, two late promoters are activated; the late transcripts are initiated at sites such that the secondary structure can not form, and translation of the late messages occurs.

MeSH Terms
Amino Acid Sequence Base Sequence Escherichia coli/enzymology,genetics Genes Genes, Viral Homeostasis Kinetics Muramidase/genetics Nucleic Acid Conformation Promoter Regions, Genetic Protein Biosynthesis T-Phages/enzymology,genetics Transcription, Genetic Tritium
Chemicals
Tritium Muramidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McPheeters D S
Christensen A
Young E T
Stormo G
Gold L
References (31)
31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.14.5813
Published
1986-07-25
Pages
5813-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311593
Subset
IM
Grants
NIAID NIH HHS · AI-09456 · United States
NIGMS NIH HHS · GM19963 · United States
NIGMS NIH HHS · GM28755 · United States
Databases
GENBANK
J02514, V00860
External Links
PubMed source DOI: 10.1093/nar/14.14.5813 PMC Full Text View journal details
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