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PMID: 3526331 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5512-6

Baum P, Furlong C, Byers B

Abstract

Saccharomyces cerevisiae strains bearing temperature-sensitive alleles of the cell division cycle gene CDC31 are specifically defective in duplication of the spindle pole body, the microtubule-organizing center of yeast. To define the function encoded by CDC31 more fully, we have isolated genomic clones of the gene by selection for complementation of a temperature-sensitive allele. The locus from which the clone was derived was marked by integration of a nutritional marker and found by meiotic mapping to cosegregate with CDC31. The polypeptide sequence of the open reading frame in the CDC31 gene was determined and compared with the sequences of other known proteins. This revealed significant homology with the calmodulins and other members of the Ca2+-binding protein family. On the basis of comparison with these related proteins, it is evident that the CDC31 gene product has at least two binding sites for Ca2+ and is also homologous with other regions of the calmodulin sequence. We propose that Ca2+ fluxes within the yeast cell play a key role in spindle pole body duplication and consequently in the organization of the microtubule arrays.

MeSH Terms
Amino Acid Sequence Base Sequence Calcium/physiology Calcium-Binding Proteins/physiology Calmodulin/physiology Cell Cycle Cloning, Molecular Fungal Proteins/physiology Microtubules/physiology,ultrastructure Mitosis Saccharomyces cerevisiae/physiology Sequence Homology, Nucleic Acid Spindle Apparatus/physiology
Chemicals
Calcium-Binding Proteins Calmodulin Fungal Proteins Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baum P
Furlong C
Byers B
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5512
Published
1986-08-00
Pages
5512-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386317
Subset
IM
Grants
NIA NIH HHS · AG-00057 · United States
NIGMS NIH HHS · GM-15253 · United States
NIGMS NIH HHS · GM-18541 · United States
Databases
GENBANK
M14078
External Links
PubMed source DOI: 10.1073/pnas.83.15.5512 PMC Full Text View journal details
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