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PMID: 3526335 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

recD: the gene for an essential third subunit of exonuclease V.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5558-62

Amundsen SK, Taylor AF, Chaudhury AM, Smith GR

Abstract

Exonuclease V (EC 3.1.11.5) of Escherichia coli, an enzyme with multiple activities promoting genetic recombination, has previously been shown to contain two polypeptides, the products of the recB and recC genes. We report here that the enzyme contains in addition a third polypeptide (alpha) with a molecular mass of about 58 kDa. The alpha polypeptide is not synthesized by a class of mutants (previously designated recB) lacking the nuclease activity of exonuclease V but retaining recombination proficiency. The gene, recD, coding for the alpha polypeptide is located near recB in the order thyA-recC-ptr-recB-recD-argA on the E. coli chromosome. The recB and recD genes appear to be governed by a common promoter to the left of recB; a weaker promoter appears to govern recD alone. In the light of these results we discuss the relation between the structure and function of the three polypeptides of exonuclease V, hereby alternatively designated RecBCD enzyme.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping Escherichia coli/enzymology,genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/genetics,isolation & purification Genes, Bacterial Genetic Complementation Test Macromolecular Substances Molecular Weight Recombination, Genetic
Chemicals
Bacterial Proteins Escherichia coli Proteins Macromolecular Substances Exodeoxyribonucleases Exodeoxyribonuclease V exodeoxyribonuclease V, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Amundsen S K
Taylor A F
Chaudhury A M
Smith G R
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5558
Published
1986-08-00
Pages
5558-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386327
Subset
IM
Grants
NIAID NIH HHS · AI 00547 · United States
NCI NIH HHS · CA 09229 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.15.5558 PMC Full Text View journal details
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