Abstract
The compound MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate)] is a potent anticonvulsant that is active after oral administration and whose mechanism of action is unknown. We have detected high-affinity (Kd = 37.2 +/- 2.7 nM) binding sites for [3H]MK-801 in rat brain membranes. These sites are heat-labile, stereoselective, and regionally specific, with the hippocampus showing the highest density of sites, followed by cerebral cortex, corpus striatum, and medulla-pons. There was no detectable binding in the cerebellum. MK-801 binding sites exhibited a novel pharmacological profile, since none of the major neurotransmitter candidates were active at these sites. The only compounds that were able to compete for [3H]MK-801 binding sites were substances known to block the responses of excitatory amino acids mediated by the N-methyl-D-aspartate (N-Me-D-Asp) receptor subtype. These comprised the dissociative anesthetics phencyclidine and ketamine and the sigma-type opioid N-allylnormetazocine (SKF 10,047). Neurophysiological studies in vitro, using a rat cortical-slice preparation, demonstrated a potent, selective, and noncompetitive antagonistic action of MK-801 on depolarizing responses to N-Me-D-Asp but not to kainate or quisqualate. The potencies of phencyclidine, ketamine, SKF 10,047, and the enantiomers of MK-801 as N-Me-D-Asp antagonists correlated closely (r = 0.99) with their potencies as inhibitors of [3H]MK-801 binding. This suggests that the MK-801 binding sites are associated with N-Me-D-Asp receptors and provides an explanation for the mechanism of action of MK-801 as an anticonvulsant.
MeSH Terms
Animals
Anticonvulsants/pharmacology
Aspartic Acid/analogs & derivatives,antagonists & inhibitors
Binding Sites
Brain/metabolism
Dibenzocycloheptenes/metabolism,pharmacology
Dizocilpine Maleate
In Vitro Techniques
Ketamine/pharmacology
Male
N-Methylaspartate
Phenazocine/analogs & derivatives,pharmacology
Phencyclidine/pharmacology
Rats
Rats, Inbred Strains
Chemicals
Anticonvulsants
Dibenzocycloheptenes
Aspartic Acid
N-Methylaspartate
Ketamine
Dizocilpine Maleate
SK&F 10047
Phenazocine
Phencyclidine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wong E H
Kemp J A
Priestley T
Knight A R
Woodruff G N
Iversen L L
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