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PMID: 3530500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The membrane-spanning segment of invariant chain (I gamma) contains a potentially cleavable signal sequence.

Cell ·Vol. 46 ·No. 7 ·1986-09-26 ·Pages 1103-12

Lipp J, Dobberstein B

Abstract

The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is replaced by the cytoplasmic protein chloramphenicol acetyltransferase (CAT), CAT becomes the exoplasmic, glycosylated domain of the resulting membrane protein I gamma CAT. Deletion of the hydrophilic cytoplasmic domain from I gamma CAT gives rise to a secreted protein from which an amino-terminal segment is cleaved, most likely by signal peptidase. We conclude that the membrane-spanning region of I gamma contains a signal sequence in its amino-terminal half and that hydrophilic residues at the amino-terminal end of a signal sequence can determine cleavage by signal peptidase.

MeSH Terms
Acetyltransferases/genetics Biological Transport Chloramphenicol O-Acetyltransferase Endopeptidases/physiology Endoplasmic Reticulum/metabolism Glycosylation HLA-D Antigens/genetics HLA-DR Antigens/genetics Humans Membrane Proteins/genetics Protein Processing, Post-Translational Protein Sorting Signals/genetics Recombinant Fusion Proteins/metabolism Serine Endopeptidases Structure-Activity Relationship
Chemicals
HLA-D Antigens HLA-DR Antigens Membrane Proteins Protein Sorting Signals Recombinant Fusion Proteins Acetyltransferases Chloramphenicol O-Acetyltransferase Endopeptidases Serine Endopeptidases type I signal peptidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lipp J
Dobberstein B
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41 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(86)90710-5
Published
1986-09-26
Pages
1103-12
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133317
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(86)90710-5 PMC Full Text View journal details
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