Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 19 ·1986-10-00 ·Pages 7462-6

Olmsted RA, Elango N, Prince GA, Murphy BR, Johnson PR, Moss B, Chanock RM, Collins PL

Abstract

A cDNA clone representing the mRNA coding sequence of the fusion glycoprotein (F) gene of human respiratory syncytial virus (RSV) was constructed and inserted into the thymidine kinase gene of vaccinia virus (WR strain) under the control of a vaccinia virus promoter. The resulting recombinant vaccinia virus, vaccinia F, expressed the F1 and F2 cleavage products (48 and 20 kDa, respectively) of the F glycoprotein in cell culture. F1 and F2 were indistinguishable from their authentic RSV counterparts with respect to glycosylation, disulfide linkage, electrophoretic mobility, cell-surface expression, and antigenic specificity. Cotton rats infected intradermally with vaccinia F developed a high titer of serum F-specific antibodies, which neutralized infectivity of RSV. This neutralizing antibody response exceeded that induced by infection of the respiratory tract with RSV and was 6-fold higher than that induced by vaccinia G, a recombinant vaccinia virus that expressed the RSV G glycoprotein gene. Immunization with vaccinia F stimulated almost complete resistance to replication of RSV in the lower respiratory tract as well as significant resistance in the upper respiratory tract. The degree of resistance conferred by vaccinia F exceeded that induced by vaccinia G.

MeSH Terms

Animals Antigens Arvicolinae/immunology Cloning, Molecular Gene Expression Regulation Neutralization Tests Respiratory Syncytial Viruses/immunology Vaccines, Synthetic Vaccinia virus/genetics Viral Fusion Proteins/genetics,immunology Viral Vaccines

Chemicals

Antigens Vaccines, Synthetic Viral Fusion Proteins Viral Vaccines

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Olmsted R A

Elango N

Prince G A

Murphy B R

Johnson P R

Moss B

Chanock R M

Collins P L

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.19.7462

Published

1986-10-00

Pages

7462-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC386738

Subset

External Links

PubMed source DOI: 10.1073/pnas.83.19.7462 PMC Full Text View journal details

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Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

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