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PMID: 3534791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.

Nucleic acids research ·Vol. 14 ·No. 19 ·1986-10-10 ·Pages 7695-703

Finch PW, Wilson RE, Brown K, Hickson ID, Emmerson PT

Abstract

The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long, which would encode a protein consisting of 962 amino acids with a calculated molecular mass of 107,719 daltons. The predicted primary structure of the protein includes a 23-residue signal sequence, cleavage of which would give rise to a mature protein of molecular mass 105,124 daltons. At its 3' end, the ptr gene overlaps the start of the recB coding sequence by 8 bases, suggesting that these genes may form part of an operon.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Base Sequence Codon Endopeptidases/analysis,genetics Escherichia coli/genetics Genes Genes, Bacterial Metalloendopeptidases Transcription, Genetic
Chemicals
Amino Acids Codon Endopeptidases Metalloendopeptidases pitrilysin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Finch P W
Wilson R E
Brown K
Hickson I D
Emmerson P T
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.19.7695
Published
1986-10-10
Pages
7695-703
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311789
Subset
IM
Databases
GENBANK
X06227
External Links
PubMed source DOI: 10.1093/nar/14.19.7695 PMC Full Text View journal details
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