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PMID: 353513 Published · ppublish English Journal Article

The recognition of mismatched base pairs in DNA by DNase I from Ustilago maydis.

Molecular & general genetics : MGG ·Vol. 161 ·No. 3 ·1978-05-31 ·Pages 245-50

Pukkila PJ

Abstract

The activity of Ustilago maydis DNase I, an enzyme implicated in genetic recombination, on DNA substrates containing unpaired or mismatched bases, was examined. The enzyme nicked supercoiled PM-2 molecules, converting these to relaxed circular and linear molecules. Discrete double stranded linear fragments smaller than unit length were also observed after digestion at high enzyme concentration. Heteroduplex molecules were constructed using phi80 bacteriophage derivatives which contained single base substitutions within the E. coli tRNA1tyr gene. Single and double stranded nicking at or near the single mismatched site was observed with three out of the five pairs of heteroduplexes.

MeSH Terms
Alleles Base Sequence DNA, Superhelical/metabolism Deoxyribonucleases/metabolism Escherichia coli Nucleic Acid Hybridization RNA, Bacterial RNA, Transfer Recombination, Genetic Ustilago/enzymology
Chemicals
DNA, Superhelical RNA, Bacterial RNA, Transfer Deoxyribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pukkila P J
References (34)
34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330997
Published
1978-05-31
Pages
245-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330997
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