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PMID: 3536483 Published · ppublish English Journal Article

The mom gene of bacteriophage Mu: the mechanism of methylation-dependent expression.

The EMBO journal ·Vol. 5 ·No. 10 ·1986-10-00 ·Pages 2719-28

Seiler A, Blöcker H, Frank R, Kahmann R

Abstract

Transcription of the DNA modification gene (mom) of bacteriophage Mu requires methylation of three GATC sites upstream of the mom promoter by the Escherichia coli deoxyadenosine methylation function (Dam). The three sites map within a 40-bp segment termed region I. Small deletions, inversions, duplications and specific point mutations have been introduced in region I. Their effect on mom expression has been studied in dam+ and dam strains. Dam-dependent expression of the mom gene requires a specific arrangement of the three GATC sites and the presence of the methylated base in at least two of the three sites. We show that mom specific modification is regulated by a host protein. The Mom function is expressed in dam strains if they are defective in one component of the methylation-instructed mismatch correction system, mutH. We suggest that the product of mutH functions as a transcriptional repressor by binding to region I.

MeSH Terms
Base Sequence Chromosome Deletion Coliphages/genetics DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Methylation Mutation Nucleic Acid Conformation Promoter Regions, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Seiler A
Blöcker H
Frank R
Kahmann R
References (51)
51 references, click to expand
  1. Analysis of the methylation-regulated Mu mom transcript.
    Cell. 1984 Jan;36(1):189-96 PMID: 6229339
  2. The mom gene of bacteriophage Mu.
    Curr Top Microbiol Immunol. 1984;108:29-47 PMID: 6232116
  3. Transcription initiation of Mu mom depends on methylation of the promoter region and a phage-coded transactivator.
    Nature. 1983 Jan 27;301(5898):344-7 PMID: 6218410
  4. Insertion mutations in the dam gene of Escherichia coli K-12.
    Mol Gen Genet. 1983;192(1-2):288-9 PMID: 6316110
  5. Mapping of the modification function of temperate phage Mu-1.
    Mol Gen Genet. 1980 Jan;177(2):351-3 PMID: 6445035
  6. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.
    J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
  7. Further characterization of a non-essential mutator gene in Escherichia coli K-12.
    J Bacteriol. 1975 Nov;124(2):650-60 PMID: 1102526
  8. Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.
    Genetics. 1954 Jul;39(4):429-39 PMID: 17247494
  9. The sequence and mom-transactivation function of the C gene of bacteriophage Mu.
    Gene. 1986;43(1-2):59-67 PMID: 3019838
  10. Prophage deletion mapping of bacteriophage Mu-1.
    Virology. 1973 Jul;54(1):93-101 PMID: 4351616
  11. Cleavage of the site-specific recombination protein gamma delta resolvase: the smaller of two fragments binds DNA specifically.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2001-5 PMID: 6326096
  12. Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.
    Cell. 1984 Oct;38(3):879-87 PMID: 6386179
  13. Regulation and expression of the bacteriophage mu mom gene: mapping of the transactivation (dad) function to the C region.
    Gene. 1985;39(1):71-6 PMID: 2934297
  14. DNA methyltransferase-dependent transcription of the phage Mu mom gene.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5518-21 PMID: 6752951
  15. Unusual modification of bacteriophage Mu DNA.
    J Virol. 1979 Nov;32(2):468-75 PMID: 159363
  16. Mechanism and regulation of Tn10 transposition.
    Cold Spring Harb Symp Quant Biol. 1984;49:235-44 PMID: 6099238
  17. Evidence that adenine methylation influences DNA-protein interactions in Escherichia coli.
    J Bacteriol. 1985 Oct;164(1):490-3 PMID: 2995323
  18. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  19. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  20. Regulation of the DNA-modification function of bacteriophage Mu.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:647-53 PMID: 6305580
  21. Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12.
    J Bacteriol. 1973 Jun;114(3):1143-50 PMID: 4576399
  22. The mom gene of bacteriophage mu: a unique regulatory scheme to control a lethal function.
    Gene. 1985;39(1):61-70 PMID: 2934296
  23. A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports.
    Nucleic Acids Res. 1983 Jul 11;11(13):4365-77 PMID: 6306587
  24. DNA modification of bacteriophage Mu-1 requires both host and bacteriophage functions.
    J Virol. 1977 Sep;23(3):825-6 PMID: 330882
  25. Repressor structure and the mechanism of positive control.
    Cell. 1983 Feb;32(2):319-25 PMID: 6218886
  26. Effect of dam methylation on the activity of the E. coli replication origin, oriC.
    EMBO J. 1985 May;4(5):1327-32 PMID: 3891330
  27. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.
    Mol Gen Genet. 1980 Apr;178(1):9-20 PMID: 6991883
  28. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  29. Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli.
    Genetics. 1983 Aug;104(4):571-82 PMID: 6225697
  30. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  31. High-frequency generalised transduction by bacteriophage T4.
    Nature. 1979 Jul 5;280(5717):80-2 PMID: 15305587
  32. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7 PMID: 6987663
  33. The DNA modification function of temperate phage Mu-1.
    Virology. 1976 Mar;70(1):17-27 PMID: 1258376
  34. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  35. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.
    Mutat Res. 1975 Apr;28(1):15-26 PMID: 167279
  36. S1 nuclease mapping of the phage Mu mom gene promoter: a model for the regulation of mom expression.
    Gene. 1984 Jul-Aug;29(1-2):185-98 PMID: 6092220
  37. Purification and characterization of the unusual deoxynucleoside, alpha-N-(9-beta-D-2'-deoxyribofuranosylpurin-6-yl)glycinamide, specified by the phage Mu modification function.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7400-4 PMID: 6369315
  38. The frameshift mutability of polA1 and recA1 derivatives of mutator strains of Escherichia coli.
    Mutat Res. 1977 Mar;42(3):443-6 PMID: 323701
  39. Efficient site-directed mutagenesis by simultaneous use of two primers.
    Nucleic Acids Res. 1983 Aug 11;11(15):5103-12 PMID: 6308572
  40. Negative supercoiling induces spontaneous unwinding of a bacterial promoter.
    EMBO J. 1985 Apr;4(4):1025-32 PMID: 2990904
  41. Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
    J Mol Biol. 1975 Mar 15;92(4):529-40 PMID: 1097703
  42. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
    Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
  43. Methyl-directed repair of DNA base-pair mismatches in vitro.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4639-43 PMID: 6308634
  44. Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli.
    EMBO J. 1985 May;4(5):1319-26 PMID: 3891329
  45. How the lambda repressor and cro work.
    Cell. 1980 Jan;19(1):1-11 PMID: 6444544
  46. Changing the DNA-binding specificity of a repressor.
    Cell. 1983 Dec;35(3 Pt 2):777-83 PMID: 6652685
  47. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  48. SOS mutator effect in E. coli mutants deficient in mismatch correction.
    EMBO J. 1984 Apr;3(4):707-12 PMID: 6233141
  49. Electron microscopic characterization of DNAs of non-defective deletion mutants of bacteriophage Mu.
    J Mol Biol. 1977 Jul 15;113(4):591-609 PMID: 894712
  50. Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:639-46 PMID: 6345072
  51. The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistance.
    Mol Gen Genet. 1979 May 4;172(2):179-84 PMID: 289892
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-10-00
Pages
2719-28
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167174
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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