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PMID: 3536807 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical localization of epidermal growth factor in rat and man.

Histochemistry ·Vol. 85 ·No. 5 ·1986-00-00 ·Pages 389-94

Poulsen SS, Nexø E, Olsen PS, Hess J, Kirkegaard P

Abstract

Epidermal growth factor (EGF) is a peptide which stimulates cell mitotic activity and differentiation, has a cytoprotective effect on the gastroduodenal mucosa, and inhibits gastric acid secretion. The immunohistochemical localization of EGF in the Brunner's glands and the submandibular glands is well documented. The localization of EGF in other tissues is still unclarified. In the present study, the immunohistochemical localization of EGF in tissues from rat, man and a 20 week human fetus were investigated. In man and rat, immunoreaction was found in the submandibular glands, the serous glands of the nasal cavity, Brunner's glands of the duodenum, the Paneth cells of the small intestine, and the tubular cells of the kidney. In the fetus EGF was found in the kidney and in the intestinal Paneth cells. Antisera raised against rat submandibular EGF did not recognize EGF in human tissues, whereas antisera against human urinary EGF worked in rat as well as man. EGF was found only in cells with an exocrine function.

MeSH Terms
Animals Brunner Glands/analysis Epidermal Growth Factor/analysis Female Fetus Humans Immunoenzyme Techniques Intestine, Small/analysis Kidney/analysis Male Nasal Mucosa/analysis Rats Rats, Inbred Strains Submandibular Gland/analysis Tissue Distribution
Chemicals
Epidermal Growth Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Poulsen S S
Nexø E
Olsen P S
Hess J
Kirkegaard P
References (22)
22 references, click to expand
  1. Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues.
    Nature. 1985 Jan 17-23;313(5999):228-31 PMID: 3871506
  2. Epidermal growth factor inhibits cysteamine-induced duodenal ulcers.
    Gastroenterology. 1983 Dec;85(6):1277-83 PMID: 6605273
  3. Immunoreactive epidermal growth factor in human amniotic fluid.
    Mt Sinai J Med. 1978 Nov-Dec;45(6):679-84 PMID: 310947
  4. Immunohistochemical localisation of urogastrone to human duodenal and submandibular glands.
    Gut. 1978 May;19(5):408-13 PMID: 350728
  5. Epidermal growth factor-urogastrone, a polypeptide acquiring hormonal status.
    Vitam Horm. 1979;37:69-110 PMID: 398091
  6. Immunohistochemical localization of nerve growth factor and epidermal growth factor in guinea pig prostate gland.
    Histochemistry. 1984;80(4):411-3 PMID: 6376431
  7. Epidermal growth factor: effects of androgens and adrenergic agents.
    Endocrinology. 1974 Sep;95(3):776-82 PMID: 4368688
  8. Isolation and structure of urogastrone and its relationship to epidermal growth factor.
    Nature. 1975 Sep 25;257(5524):325-7 PMID: 1161035
  9. Radioimmunoassay of human epidermal growth factor (urogastrone).
    J Clin Endocrinol Metab. 1977 Dec;45(6):1144-53 PMID: 304067
  10. Role of submandibular saliva and epidermal growth factor in gastric cytoprotection.
    Gastroenterology. 1984 Jul;87(1):103-8 PMID: 6609862
  11. Epidermal growth factor-like material in rat submandibular gland.
    Am J Anat. 1979 Sep;156(1):83-9 PMID: 391019
  12. Immunocytochemical localization of epidermal growth factor in mouse submandibular gland.
    J Histochem Cytochem. 1977 Sep;25(9):1027-35 PMID: 333017
  13. The identification of urogastrone in serum, saliva, and gastric juice.
    Gastroenterology. 1979 Aug;77(2):313-8 PMID: 312747
  14. Cellular localisation of human urogastrone/epidermal growth factor.
    Nature. 1978 Feb 2;271(5644):466-7 PMID: 342970
  15. Gastric cytoprotection by epidermal growth factor. Role of endogenous prostaglandins and DNA synthesis.
    Gastroenterology. 1981 Sep;81(3):438-43 PMID: 7018988
  16. Immunocytochemical localization of human epidermal growth factor/urogastrone in several human tissues.
    J Histochem Cytochem. 1985 Apr;33(4):315-22 PMID: 3884705
  17. Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects.
    Gut. 1975 Nov;16(11):887-93 PMID: 1104412
  18. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  19. Quantitation of epidermal growth factor in the rat. Identification and partial characterization of duodenal EGF.
    Scand J Gastroenterol. 1983 Sep;18(6):771-6 PMID: 6608137
  20. Epidermal growth factor immunoreactive material in the central nervous system: location and development.
    Science. 1984 Jun 8;224(4653):1107-9 PMID: 6144184
  21. Renal origin of rat urinary epidermal growth factor.
    Regul Pept. 1984 Dec;10(1):37-45 PMID: 6335757
  22. Epidermal growth factor (urogastrone) in human tissues.
    J Clin Endocrinol Metab. 1979 Apr;48(4):667-72 PMID: 219006
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00982668
Published
1986-00-00
Pages
389-94
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00982668
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