Home LiteratureArticle Details
PMID: 3537692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antibodies to the ras gene product inhibit adenylate cyclase and accelerate progesterone-induced cell division in Xenopus laevis oocytes.

Molecular and cellular biology ·Vol. 6 ·No. 2 ·1986-02-00 ·Pages 719-22

Sadler SE, Schechter AL, Tabin CJ, Maller JL

Abstract

Microinjection of monoclonal antibodies (lines 238, 172, and 259) directed against the ras gene product, p21, into Xenopus laevis oocytes accelerated progesterone-induced germinal vesicle breakdown. Antibody 238 had the greatest effect on the acceleration of progesterone-induced oocyte maturation, and this effect was correlated with in vitro inhibition of adenylate cyclase (EC 4.6.1.1) activity in a concentration-dependent manner. Inhibition of adenylate cyclase by antibody 238 was also measured in membranes prepared from oocytes pretreated with either cholera toxin or pertussis toxin. These results suggest a role for the ras gene product in the regulation of vertebrate cell adenylate cyclase activity.

MeSH Terms
Adenylyl Cyclase Inhibitors Adenylyl Cyclases/genetics Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Division/drug effects Female Genes Microinjections Oocytes/cytology,drug effects,enzymology Progesterone/pharmacology Proto-Oncogene Proteins/genetics,immunology Proto-Oncogene Proteins p21(ras) Proto-Oncogenes Xenopus
Chemicals
Adenylyl Cyclase Inhibitors Antibodies, Monoclonal Antigen-Antibody Complex Proto-Oncogene Proteins Progesterone Proto-Oncogene Proteins p21(ras) Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sadler S E
Schechter A L
Tabin C J
Maller J L
References (26)
26 references, click to expand
  1. Two distinct adenosine-sensitive sites on adenylate cyclase.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5482-6 PMID: 271970
  2. Inhibition of Xenopus oocyte adenylate cyclase by progesterone: a novel mechanism of action.
    Adv Cyclic Nucleotide Protein Phosphorylation Res. 1985;19:179-94 PMID: 3159186
  3. p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation.
    J Virol. 1979 Aug;31(2):546-6 PMID: 225570
  4. Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9 PMID: 228288
  5. Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1980 Apr;19(4):1005-14 PMID: 6247068
  6. Identification of a normal vertebrate cell protein related to the p21 src of Harvey murine sarcoma virus.
    Virology. 1980 Oct 30;106(2):292-300 PMID: 6254252
  7. Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.
    J Biol Chem. 1981 Jun 25;256(12):6368-73 PMID: 7240211
  8. Progesterone inhibits membrane-bound adenylate cyclase in Xenopus laevis oocytes.
    Nature. 1981 Jul 16;292(5820):255-7 PMID: 7195984
  9. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.
    Nature. 1981 Aug 6;292(5823):506-11 PMID: 6265801
  10. Adenosine receptors and the regulation of adenylate cyclase.
    Adv Cyclic Nucleotide Res. 1981;14:199-214 PMID: 6269379
  11. Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus.
    J Virol. 1982 Apr;42(1):253-61 PMID: 6283143
  12. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.
    J Virol. 1982 Jul;43(1):294-304 PMID: 6287003
  13. Comparative biochemical properties of p21 ras molecules coded for by viral and cellular ras genes.
    J Virol. 1982 Nov;44(2):509-19 PMID: 6292515
  14. Inhibition of Xenopus oocyte adenylate cyclase by progesterone and 2',5'-dideoxyadenosine is associated with slowing of guanine nucleotide exchange.
    J Biol Chem. 1983 Jul 10;258(13):7935-41 PMID: 6305962
  15. G proteins and dual control of adenylate cyclase.
    Cell. 1984 Mar;36(3):577-9 PMID: 6321035
  16. The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
    Dev Biol. 1984 Mar;102(1):79-89 PMID: 6365656
  17. Activation of ras genes in human tumors does not affect localization, modification, or nucleotide binding properties of p21.
    Cell. 1984 May;37(1):151-8 PMID: 6609772
  18. Comparative biochemical properties of normal and activated human ras p21 protein.
    Nature. 1984 Aug 23-29;310(5979):644-9 PMID: 6147754
  19. Oocyte adenylyl cyclase contains Ni, yet the guanine nucleotide-dependent inhibition by progesterone is not sensitive to pertussis toxin.
    FEBS Lett. 1984 Sep 17;175(1):25-30 PMID: 6434346
  20. The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activity.
    Nature. 1984 Sep 20-26;311(5983):273-5 PMID: 6148703
  21. Progesterone inhibition of Xenopus oocyte adenylate cyclase is not mediated via the Bordetella pertussis toxin substrate.
    Mol Pharmacol. 1984 Nov;26(3):526-31 PMID: 6541752
  22. Homologies between signal transducing G proteins and ras gene products.
    Science. 1984 Nov 16;226(4676):860-2 PMID: 6436980
  23. Does the guanine nucleotide regulatory protein Ni mediate progesterone inhibition of Xenopus oocyte adenylate cyclase?
    EMBO J. 1984 Nov;3(11):2653-7 PMID: 6439557
  24. In yeast, RAS proteins are controlling elements of adenylate cyclase.
    Cell. 1985 Jan;40(1):27-36 PMID: 2981630
  25. Primary structure of the alpha-subunit of transducin and its relationship to ras proteins.
    Nature. 1985 May 16-22;315(6016):242-5 PMID: 3923359
  26. Identification of a sarcoma virus-coded phosphoprotein in nonproducer cells transformed by Kirsten or Harvey murine sarcoma virus.
    Virology. 1979 Jul 15;96(1):64-79 PMID: 223311
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.2.719-722.1986
Published
1986-02-00
Pages
719-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367565
Subset
IM
Grants
NIGMS NIH HHS · GM26743 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.2.719-722.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2